Spatiotemporal dynamics of actin-rich adhesion microdomains: influence of substrate flexibility

Journal of Cell Science
Olivier CollinEmmanuelle Planus

Abstract

In this study we analyse the formation and dynamics of specific actin-rich structures called podosomes. Podosomes are very dynamic punctual adhesion sites tightly linked to the actin cytoskeleton. Mechanical properties of substrates are emerging as important physical modulators of anchorage-dependent processes involved in the cellular response. We investigate the influence of substrate flexibility on the dynamic properties of podosomes. We used mouse NIH-3T3 fibroblasts, transfected with GFP-actin and cultured on polyacrylamide collagen-coated substrates of varying stiffness. Static and dynamic features of cell morphologies associated with an optical flow analysis of the dynamics of podosomes revealed that: (1) they have constant structural properties, i.e. their shape factor and width do not change with the substrate flexibility; (2) the lifespan of podosomes and mean minimum distance between them depend on the substrate flexibility; (3) there is a variation in the displacement speed of the rosette of podosomes. Moreover, the rosettes sometimes appear as periodically emergent F-actin structures, which suggests that a two-level self-organisation process may drive first, the formation of clusters of podosomes and second, the org...Continue Reading

References

Jun 1, 1978·Nature·J Folkman, A Moscona
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·T David-Pfeuty, S J Singer
Feb 9, 1996·Cell·D A Lauffenburger, A F Horwitz
Jul 1, 1995·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·W AltH W Kaiser
Jan 1, 1996·Annual Review of Cell and Developmental Biology·K Burridge, M Chrzanowska-Wodnicka
Jan 1, 1997·Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie·H L Guyader, C Hyver
May 30, 1997·Science·C S ChenD E Ingber
Jan 1, 1997·Cell Motility and the Cytoskeleton·S G BabbS S Lim
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·R J Pelham, Y l Wang
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·R D MullinsT D Pollard
Apr 21, 1999·Current Opinion in Cell Biology·S M Schoenwaelder, K Burridge
May 7, 1999·IEEE Transactions on Bio-medical Engineering·F GermainP Tracqui
Jun 22, 1999·Current Biology : CB·K RottnerJ V Small
Jan 5, 2000·Cell Motility and the Cytoskeleton·V C MuderaR A Brown
Feb 23, 2000·The Journal of Cell Biology·M ChellaiahK A Hruska
Apr 27, 2000·Nature Cell Biology·E ZamirB Geiger
Jun 27, 2000·Biophysical Journal·C M LoY L Wang
Mar 7, 2001·Biology of the Cell·V Petit, J P Thiery
Sep 21, 2001·Journal of Neuroscience Methods·D ZoccolanV Torre
Nov 21, 2001·Nature Reviews. Molecular Cell Biology·B GeigerK M Yamada
Nov 27, 2001·Science·E CukiermanK M Yamada
Apr 16, 2002·The International Journal of Biochemistry & Cell Biology·Irina KaverinaJ Victor Small
Jul 27, 2002·Annual Review of Cell and Developmental Biology·Matthew D Welch, R Dyche Mullins
Mar 13, 2003·Journal of Muscle Research and Cell Motility·E Michael Ostap
May 21, 2003·The Journal of Cell Biology·James G EvansPaul Matsudaira
Jul 3, 2003·Trends in Cell Biology·Stefan Linder, Martin Aepfelbacher
Jul 16, 2003·Biochemical and Biophysical Research Communications·Christopher S ChenDonald E Ingber
Sep 16, 2003·Molecular and Cellular Biology·Violaine MoreauElisabeth Génot
Dec 17, 2003·Journal of Cell Science·Gerald Burgstaller, Mario Gimona
Jan 9, 2004·Current Biology : CB·Till BretschneiderGünther Gerisch
Mar 26, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·I Rita HurstL Shannon Holliday
Apr 20, 2004·Experimental Cell Research·Laura SpinardiPier Carlo Marchisio
Aug 4, 2004·The Journal of Cell Biology·Rebecca L BerdeauxG Steven Martin
Aug 6, 2004·Bulletin of Mathematical Biology·A StéphanouP Tracqui
Sep 16, 2004·Nature Reviews. Molecular Cell Biology·Roberto BuccioneMark A McNiven

❮ Previous
Next ❯

Citations

Jan 22, 2008·Annals of Biomedical Engineering·Sheng-Yang LeeHaw-Ming Huang
Oct 10, 2009·Annals of Biomedical Engineering·Stephanie Nemir, Jennifer L West
Mar 14, 2008·The Journal of Biological Chemistry·Bhavik DesaiMeenakshi A Chellaiah
Oct 1, 2008·Physical Biology·F ChamarauxB Fourcade
Nov 30, 2007·Molecular Biology of the Cell·Cédric BadowskiMarc R Block
Nov 6, 2007·Molecular Biology of the Cell·Olivier DestaingRoland Baron
Jul 24, 2008·Annual Review of Biomedical Engineering·Yingxiao WangShu Chien
Aug 21, 2009·Journal of Cell Science·Corinne Albiges-RizoMarc R Block
Jun 2, 2010·HFSP Journal·Britta Schroth-DiezGünther Gerisch
Dec 29, 2011·Cellular and Molecular Life Sciences : CMLS·Koen van den DriesCarl G Figdor
Aug 2, 2011·Annual Review of Cell and Developmental Biology·Stefan LinderMirko Himmel
Jul 9, 2011·Molecular Biology of the Cell·Shiqiong HuJean-Christophe Géminard
Apr 20, 2013·European Journal of Cell Biology·Heiani TouaitahuataGeraldine Pawlak
Jul 25, 2012·European Journal of Cell Biology·Lindsy R Boateng, Anna Huttenlocher
Jun 8, 2012·European Journal of Cell Biology·Lucie BrissonSébastien Roger
Jun 23, 2012·European Journal of Cell Biology·Pasquale CerveroStefan Linder
Oct 18, 2011·Journal of Colloid and Interface Science·Jianyong HuangJing Fang
Aug 4, 2009·Biomaterials·Christopher G WilsonEdie C Goldsmith
May 5, 2009·Medical Hypotheses·Vic NorrisDenis Gruber
Dec 15, 2015·Experimental Cell Research·Stefan Linder, Christiane Wiesner
Jun 25, 2009·Chembiochem : a European Journal of Chemical Biology·Eun-Ju LeeMuhammad N Yousaf
Apr 25, 2006·Cell Motility and the Cytoskeleton·Sophie FéréolEmmanuelle Planus
Dec 25, 2008·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·A KamgouéP Tracqui
Oct 31, 2012·Biology of the Cell·Amélie JuinFrédéric Saltel
Jul 19, 2012·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Viviana CremascoRoberta Faccio
Dec 15, 2012·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Takumi MatsumotoSakae Tanaka
Jun 28, 2013·Cytoskeleton·Hannah SchachtnerLaura M Machesky
Oct 17, 2014·Immunological Reviews·Isabelle Maridonneau-Parini
Aug 26, 2006·Current Opinion in Cell Biology·Alexander BershadskyBenjamin Geiger
Jun 27, 2014·Small GTPases·Pirjo SpuulElisabeth Génot
Apr 9, 2016·Trends in Cell Biology·FuiBoon KaiValerie M Weaver
Apr 14, 2016·Macromolecular Bioscience·E Rodríguez-PérezC Martínez-Ramos
Jul 27, 2014·European Journal of Cell Biology·Radoslav JanoštiakDaniel Rösel
Jun 2, 2015·The International Journal of Biochemistry & Cell Biology·Veronique VeillatElisabeth Génot
Jan 15, 2014·Acta Biomaterialia·Rachel J Jerrell, Aron Parekh
Sep 2, 2008·Current Biology : CB·Olivier CollinNing Wang
May 10, 2011·Current Opinion in Cell Biology·Olivier DestaingCorinne Albiges-Rizo
Jul 8, 2010·European Journal of Cell Biology·Frédéric SaltelElisabeth Génot
Oct 11, 2014·Cellular and Molecular Life Sciences : CMLS·Stefan Linder, Christiane Wiesner
Jul 10, 2016·Matrix Biology : Journal of the International Society for Matrix Biology·Sanela MrkonjicCorinne Albiges-Rizo
Dec 28, 2016·Matrix Biology : Journal of the International Society for Matrix Biology·Tammy T ChangValerie M Weaver
Apr 1, 2016·Journal of Cell Science·Julie Di MartinoFrederic Saltel

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Anna LabernadieGuillaume M Charrière
Current Biology : CB
Nelson R AlexanderAlissa M Weaver
Trends in Cell Biology
Stefan Linder, M Aepfelbacher
© 2022 Meta ULC. All rights reserved