Spatiotemporal expression of connexin 39 and -43 during myoblast differentiation in cultured cells and in the mouse embryo

Cell Communication & Adhesion
Julia von MaltzahnK Willecke

Abstract

Connexin39 (Cx39) and connexin43 (Cx43) are known to be expressed during development of skeletal muscles. Here we have compared the expression pattern of both connexins during differentiation of established C(2)C(12) mouse myoblasts and in the mouse embryo. Cx43 is highly abundant in undifferentiated myoblasts, but no Cx39 protein was detected in these cells. Upon differentiation into myotubes, Cx39 expression increased. The consecutive expression of these connexins was also observed in the mouse embryo. Cx39 and Cx43 were found in different plaques in accordance with the notion that Cx43 is exclusively expressed in myoblasts and Cx39 in myotubes. Thus, differentiating C(2)C(12) cells in culture can serve to study the involvement of gap junctions in myogenesis, since expression of corresponding Cx39 and Cx43 proteins appears to be very similar as in the mouse embryo.

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Oct 7, 2004·Journal of Cell Science·Julia von MaltzahnGoran Söhl

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Citations

Nov 30, 2013·Nature Communications·Julia von MaltzahnMichael A Rudnicki
Oct 14, 2009·The Journal of Biological Chemistry·Sonja BuvinicEnrique Jaimovich
Jul 19, 2013·Tissue Engineering. Part a·Jill M ZemianekThomas B Shea
Dec 22, 2015·Seminars in Cell & Developmental Biology·Peter A Merrifield, Dale W Laird
Aug 22, 2014·Mediators of Inflammation·Ieva AntanavičiūtėVytenis Arvydas Skeberdis
Jun 19, 2015·Cellular and Molecular Life Sciences : CMLS·Juan C SáezChristopher P Cardozo
Jan 29, 2011·Experimental Cell Research·Julia von MaltzahnKlaus Willecke
Mar 5, 2013·Trends in Molecular Medicine·Robert C LyonFarah Sheikh
Dec 20, 2017·Nature Reviews. Rheumatology·Henry J DonahueDamian C Genetos
Jul 9, 2010·Cellular and Molecular Life Sciences : CMLS·Elisabetta MeacciLucia Formigli
Jan 9, 2009·American Journal of Physiology. Cell Physiology·A Trovato-SalinaroG Mudò
Feb 15, 2021·Translational Research : the Journal of Laboratory and Clinical Medicine·Shlok JindalGopinath Packirisamy

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