Spatiotemporal magnetic fields enhance cytosolic Ca2+ levels and induce actin polymerization via activation of voltage-gated sodium channels in skeletal muscle cells

Biomaterials
Mónica Rubio AyalaThomas Simmet

Abstract

Cellular function is modulated by the electric membrane potential controlling intracellular physiology and signal propagation from a motor neuron to a muscle fiber resulting in muscle contraction. Unlike electric fields, magnetic fields are not attenuated by biological materials and penetrate deep into the tissue. We used complex spatiotemporal magnetic fields (17-70 mT) to control intracellular signaling in skeletal muscle cells. By changing different parameters of the alternating magnetic field (amplitude, inversion time, rotation frequency), we induced transient depolarization of cellular membranes leading to i) Na+ influx through voltage-gated sodium channels (VGSC), ii) cytosolic calcium increase, and iii) VGSC- and ryanodine receptor-dependent increase of actin polymerization. The ion fluxes occurred only, when the field was applied and returned to baseline after the field was turned off. The 30-s-activation-cycle could be repeated without any loss of signal intensity. By contrast, static magnetic fields of the same strength exhibited no effect on myotube Ca2+ levels. Mathematical modeling suggested a role for the alternating magnetic field-induced eddy current, which mediates a local change in the membrane potential trig...Continue Reading

Citations

Jun 26, 2018·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Vitalii ZablotskiiAlexandr Dejneka
Sep 15, 2020·Advanced Biosystems·N CastroS Lanceros-Mendez
Jan 17, 2020·Bioelectromagnetics·Alyaa I AldebsJaime E Ramirez-Vick
Jan 8, 2021·Biological Trace Element Research·Shenghang WangPeng Shang
Aug 17, 2021·Electromagnetic Biology and Medicine·Seiya Hayashi, Makiko Kakikawa

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