Spatiotemporal messenger ribonucleic acid expression of ovarian tissue inhibitors of metalloproteinases throughout the rat estrous cycle

Endocrinology
Kristen S SimpsonT E Curry

Abstract

The tissue inhibitors of metalloproteinases (TIMPs) within the ovary closely regulate the matrix metalloproteinases, enzymes capable of degrading components of the extracellular matrix. The purpose of this study was to examine the spatial and temporal messenger RNA (mRNA) expression of the TIMPs in the ovaries of normally cycling rats. Ovaries were collected at 1100 h on each day of the 4-day estrous cycle, and TIMP mRNA expression was examined by Northern blot, RT-PCR, or in situ hybridization. TIMP-1 mRNA levels were significantly higher on estrus than on any other day. Although the 1.0-kb TIMP-2 transcript did not change across the cycle, the 3.5-kb transcript decreased significantly between metestrus and diestrus. Expression of TIMP-3 mRNA decreased significantly between proestrus and estrus. TIMP-1, TIMP-2, and TIMP-3 mRNAs were primarily localized to the theca, stroma, and corpora lutea (CL) on all days of the cycle, but with distinct cyclic changes. Thecal expression of TIMP-1 and TIMP-2 mRNAs was especially high immediately before and after ovulation. TIMP-1 and TIMP-3 mRNAs, which were low to undetectable in the granulosa cells of preovulatory follicles, were greatly increased in the luteinizing cells of newly forming ...Continue Reading

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Citations

Mar 11, 2005·Biology of Reproduction·A McDonnel Smedts, T E Curry
Dec 21, 2016·Journal of Comparative Pathology·M B PeraltaM M L Velázquez
Sep 19, 2002·Molecular Reproduction and Development·Qing-Lei LiCheng Zhu
Oct 10, 2014·Journal of Ovarian Research·Juneo Freitas SilvaRogéria Serakides

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