Spatiotemporal microbial single-cell analysis using a high-throughput microfluidics cultivation platform

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
Alexander GrünbergerDietrich Kohlheyer

Abstract

Cell-to-cell heterogeneity typically evolves due to a manifold of biological and environmental factors and special phenotypes are often relevant for the fate of the whole population but challenging to detect during conventional analysis. We demonstrate a microfluidic single-cell cultivation platform that incorporates several hundred growth chambers, in which isogenic bacteria microcolonies growing in cell monolayers are tracked by automated time-lapse microscopy with spatiotemporal resolution. The device was not explicitly developed for a specific organism, but has a very generic configuration suitable for various different microbial organisms. In the present study, we analyzed Corynebacterium glutamicum microcolonies, thereby generating complete lineage trees and detailed single-cell data on division behavior and morphology in order to demonstrate the platform's overall capabilities. Furthermore, the occurrence of spontaneously induced stress in individual C. glutamicum cells was investigated by analyzing strains with genetically encoded reporter systems and optically visualizing SOS response. The experiments revealed spontaneous SOS induction in the absence of any external trigger comparable to results obtained by flow cytome...Continue Reading

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Citations

Aug 6, 2016·Nucleic Acids Research·Eugen PfeiferJulia Frunzke
Oct 22, 2016·ACS Chemical Biology·Peter M KusenJörg Pietruszka
Oct 30, 2016·Metabolic Engineering·Songyuan LiAlex Toftgaard Nielsen
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Jul 27, 2016·Engineering in Life Sciences·Alexander GrünbergerStephan Noack
Sep 6, 2019·BMC Bioinformatics·Christian Carsten SachsKatharina Nöh
Nov 11, 2017·FEMS Microbiology Letters·Holger MorschettMarco Oldiges
Mar 16, 2018·Bioprocess and Biosystems Engineering·Anna-Lena Heins, Dirk Weuster-Botz
Nov 18, 2020·Biotechnology and Bioengineering·Julian SchmitzAlexander Grünberger
Jan 8, 2021·Biotechnology and Bioengineering·Berin OzdalgicSavas Tasoglu
Dec 8, 2020·Frontiers in Bioengineering and Biotechnology·Vera OrtseifenAlexander Grünberger
Jan 5, 2021·Frontiers in Bioengineering and Biotechnology·Naia Risager WrightNikolaus Sonnenschein
Jun 3, 2021·ACS Synthetic Biology·Alina BurmeisterAlexander Grünberger
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Sep 8, 2021·Microbial Cell Factories·Patrick LenzAndreas Knapp
Sep 27, 2020·Nature Communications·Dennis Della CorteJan Marienhagen
Mar 20, 2018·New Biotechnology·Eugen KaganovitchDietrich Kohlheyer

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