Spatiotemporal norepinephrine mapping using a high-density CMOS microelectrode array

Lab on a Chip
John B WydallisCharles S Henry

Abstract

A high-density amperometric electrode array containing 8192 individually addressable platinum working electrodes with an integrated potentiostat fabricated using Complementary Metal Oxide Semiconductor (CMOS) processes is reported. The array was designed to enable electrochemical imaging of chemical gradients with high spatiotemporal resolution. Electrodes are arranged over a 2 mm × 2 mm surface area into 64 subarrays consisting of 128 individual Pt working electrodes as well as Pt pseudo-reference and auxiliary electrodes. Amperometric measurements of norepinephrine in tissue culture media were used to demonstrate the ability of the array to measure concentration gradients in complex media. Poly(dimethylsiloxane) microfluidics were incorporated to control the chemical concentrations in time and space, and the electrochemical response at each electrode was monitored to generate electrochemical heat maps, demonstrating the array's imaging capabilities. A temporal resolution of 10 ms can be achieved by simultaneously monitoring a single subarray of 128 electrodes. The entire 2 mm × 2 mm area can be electrochemically imaged in 64 seconds by cycling through all subarrays at a rate of 1 Hz per subarray. Monitoring diffusional transp...Continue Reading

References

Jan 1, 1992·Lasers in Surgery and Medicine·K T SchomackerT F Deutsch
Jun 17, 2000·Progress in Neurobiology·C KöbbertS Thanos
Jul 6, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R A Firtel, C Y Chung
May 21, 2003·Proceedings of the National Academy of Sciences of the United States of America·Warren R ZipfelWatt W Webb
Jul 23, 2003·Cancer·S David Nathanson
May 27, 2004·Nature Neuroscience·William J RosoffGeoffrey J Goodhill
Aug 17, 2004·Biosensors & Bioelectronics·F HeerH Baltes
Jul 21, 2006·Analytical and Bioanalytical Chemistry·Shigeru AmemiyaDarrick A Gross
Jun 2, 2007·Journal of Neuroscience Methods·S HafizovicA Hierlemann
Jan 9, 2009·Current Pharmaceutical Design·Yasuyuki AmohRobert M Hoffman
Feb 27, 2010·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Angus C GreyKevin L Schey
Jun 4, 2010·Analytical and Bioanalytical Chemistry·Shencheng GeChristy L Haynes
Nov 10, 2010·Cellular and Molecular Neurobiology·Nathalie C Guérineau, Michel G Desarménien
Dec 1, 1998·Analytical Chemistry·D C DuffyG M Whitesides
Dec 23, 2011·Annals of Biomedical Engineering·Beum Jun Kim, Mingming Wu
Apr 12, 2012·Journal of Neurophysiology·David JäckelAndreas Hierlemann
Nov 23, 2012·Lab on a Chip·Meghan M MensackCharles S Henry
Feb 13, 2013·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Tomokazu Matsue
Apr 23, 2013·Analytica Chimica Acta·Stefan BergnerFrank-Michael Matysik
May 7, 2013·Biosensors & Bioelectronics·Mustafa SenTomokazu Matsue
May 31, 2014·IEEE Transactions on Neural Systems and Rehabilitation Engineering : a Publication of the IEEE Engineering in Medicine and Biology Society·S R I GabranRaafat R Mansour
Jul 19, 2014·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Björn L D M Brücher, Ijaz S Jamall

❮ Previous
Next ❯

Citations

Sep 14, 2016·Biosensors & Bioelectronics·Lei WangTom Chen
Jan 17, 2016·Journal of Proteomics·Carlos Sánchez-OvejeroRaúl Manzano-Román
Apr 27, 2018·Lab on a Chip·Ian C McLeanCharles S Henry
Mar 1, 2018·Annual Review of Analytical Chemistry·Hunter J Sismaet, Edgar D Goluch
Oct 12, 2018·Journal of Neuroendocrinology·Luke A Schwerdtfeger, Stuart A Tobet
Jun 15, 2018·Chemical Science·Tzu-En LinAndreas Lesch
Dec 30, 2020·Biosensors & Bioelectronics·Longqian XuHanbin Ma
Nov 2, 2017·Analytical Chemistry·Yusuke KannoTomokazu Matsue

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.