Spatiotemporal profiles of arginine vasopressin transcription in cultured suprachiasmatic nucleus

The European Journal of Neuroscience
Tomoko YoshikawaKen-Ichi Honma

Abstract

Arginine vasopressin (AVP), a major neuropeptide in the suprachiasmatic nucleus (SCN), is postulated to mediate the output of the circadian oscillation. Mice carrying a reporter gene of AVP transcription (AVP(ELuc)) were produced by knocking-in a cDNA of Emerald-luciferase (ELuc) in the translational initiation site. Homozygous mice did not survive beyond postnatal day 7. Using the heterozygous (AVP(ELuc/+)) mice, a bioluminescence reporter system was developed that enabled to monitor AVP transcription through AVP-ELuc measurement in real time for more than 10 cycles in the cultured brain slice. AVP(ELuc/+) mice showed circadian behaviour rhythms and light responsiveness indistinguishable from those of the wild-type. Robust circadian rhythms in AVP-ELuc were detected in the cultured SCN slice at a single cell as well as tissue levels. The circadian rhythm of the whole SCN slice was stable, with the peak at the mid-light phase of a light-dark cycle, while that of a single cell was more variable. By comparison, rhythmicity in the paraventricular nucleus and supraoptic nucleus in the hypothalamus was unstable and damped rapidly. Spatiotemporal profiles of AVP expression at the pixel level revealed significant circadian rhythms in ...Continue Reading

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Citations

May 8, 2016·The Journal of Endocrinology·Jennifer A Evans
Mar 9, 2017·Proceedings of the National Academy of Sciences of the United States of America·Ryosuke EnokiKen-Ichi Honma
Feb 6, 2017·Scientific Reports·Ryosuke EnokiKen-Ichi Honma
Jun 23, 2018·The Journal of Comparative Neurology·Joseph L BedontJennifer A Evans
Jun 11, 2016·Nature Communications·Toshiyuki HamadaKen-Ichi Honma
Apr 19, 2017·Proceedings of the National Academy of Sciences of the United States of America·Daisuke OnoKen-Ichi Honma
Sep 20, 2018·Proceedings of the National Academy of Sciences of the United States of America·Yu-Er WuSato Honma
Dec 12, 2018·PLoS Computational Biology·Isao T TokudaHanspeter Herzel
Jul 3, 2019·Communications Biology·Daisuke OnoSato Honma
Sep 19, 2017·The European Journal of Neuroscience·Jihwan Myung, Scott D Pauls
Jan 12, 2020·Communications Biology·Daisuke OnoSato Honma
May 4, 2021·Frontiers in Neuroscience·Daisuke OnoSato Honma

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