Spatiotemporal regulation of a Legionella pneumophila T4SS substrate by the metaeffector SidJ

PLoS Pathogens
Kwang Cheol JeongJoseph P Vogel

Abstract

Modulation of host cell function is vital for intracellular pathogens to survive and replicate within host cells. Most commonly, these pathogens utilize specialized secretion systems to inject substrates (also called effector proteins) that function as toxins within host cells. Since it would be detrimental for an intracellular pathogen to immediately kill its host cell, it is essential that secreted toxins be inactivated or degraded after they have served their purpose. The pathogen Legionella pneumophila represents an ideal system to study interactions between toxins as it survives within host cells for approximately a day and its Dot/Icm type IVB secretion system (T4SS) injects a vast number of toxins. Previously we reported that the Dot/Icm substrates SidE, SdeA, SdeB, and SdeC (known as the SidE family of effectors) are secreted into host cells, where they localize to the cytoplasmic face of the Legionella containing vacuole (LCV) in the early stages of infection. SidJ, another effector that is unrelated to the SidE family, is also encoded in the sdeC-sdeA locus. Interestingly, while over-expression of SidE family proteins in a wild type Legionella strain has no effect, we found that their over-expression in a ∆sidJ mutant...Continue Reading

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Datasets Mentioned

BETA
GM130

Methods Mentioned

BETA
GTPase
transfecting
transfection
fluorescence microscopy
Assay
GTPases
transfections
ELISA

Software Mentioned

Clustal
ClustalW Guide Tree
SidJ
BlastP
SAS
Phyre

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