PMID: 8585994Nov 1, 1995Paper

Spatiotemporal relationships between a novel Drosophila stripe expressing gene and known segmentation genes by simultaneous visualization of transcript patterns

Chromosoma
C Hartmann, H Jäckle

Abstract

We describe the cloning of the Drosophila transcription unit odd-striped (ost), which maps close to the P-element insertion site of the enhancer-trap line 05279. In order rapidly to gain information on its chromosomal localization, and to determine the spatial and temporal expression patterns of ost in whole-mount embryos, we established a double-label in situ hybridization protocol to localize two different DNA or RNA sequences simultaneously. The double-label in situ hybridization method involves digoxigenin- and biotin-labeled DNA probes that are processed to result in blue alkaline phosphatase and brown peroxidase reaction products, respectively. Using reference probes as internal standards, we show that the ost transcription unit is located within the cytogenetic band interval 89A1,2 on the right arm of the third chromosome, and that it exerts diagnostic segmentation gene expression patterns in the embryo. The ost transcripts are initially expressed in an anterior cap at the blastoderm stage, followed by a transient pair-rule gene expression pattern, which eventually changes into a segmental polarity gene pattern at gastrulation. Our results establish detailed spatial and temporal relationships between ost expression and t...Continue Reading

References

Jan 24, 1992·Cell·W McGinnis, R Krumlauf
Jan 24, 1992·Cell·D St Johnston, C Nüsslein-Volhard
Oct 30, 1980·Nature·C Nüsslein-Volhard, E Wieschaus
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·P R Langer-SaferD C Ward
Jan 1, 1995·Trends in Genetics : TIG·D Tautz, R J Sommer
Aug 1, 1994·Trends in Genetics : TIG·G Hauptmann, T Gerster
Aug 1, 1993·Current Opinion in Genetics & Development·M Noll
Sep 1, 1984·Wilhelm Roux's Archives of Developmental Biology·C Nüsslein-VolhardH Kluding
Sep 1, 1984·Wilhelm Roux's Archives of Developmental Biology·G JürgensH Kluding

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Citations

Feb 12, 1998·Mechanisms of Development·G VorbrüggenH Jäckle
Feb 11, 1998·Journal of Molecular Biology·B C PerssonG Vorbrüggen
Jun 15, 1997·Developmental Biology·J P ForjanicM González Gaitán

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