SPE-HPLC method for the determination and pharmacokinetic studies on paeoniflorin in rat serum after oral administration of traditional Chinese medicinal preparation Guan-Xin-Er-Hao decoction

Journal of Pharmaceutical and Biomedical Analysis
Guan YeDean Guo


A new HPLC method for the determination of paeoniflorin in rat serum with solid-phase extraction (SPE) for preconcentration is introduced. Paeoniflorin and an internal standard (pentoxifylline) were extracted from serum by means of SPE using cartridges with octadecyl chemically bound phase. The HPLC separation was then performed on a reversed-phase C(18) column using acetonitrile-water (18:82, v/v) as eluting solvent system, and UV detection at 230 nm to measure the analyte with a limit of quantitation about 10 ng ml(-1). The calibration curve for paeoniflorin was linear (r=0.9938) in the concentration range of 10-1200 ng ml(-1), both intra- and inter-day precision of the paeoniflorin were determined and their coefficience of variation did not exceed 10%. The validated method has been successfully applied for pharmacokinetic studies of paeoniflorin from rat serum after oral administration of Guan-Xin-Er-Hao decoction.


Jan 19, 2010·Chinese Journal of Integrative Medicine·Feng Qin, Xi Huang
Jun 7, 2005·Journal of Pharmaceutical and Biomedical Analysis·Rong-Xia LiuDe-An Guo
Mar 26, 2005·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Gang ChenPengyuan Yang
Apr 14, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Fei FeiGuangji Wang
Nov 26, 2010·Journal of Pharmaceutical and Biomedical Analysis·Xue QiaoDe-an Guo
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Mar 27, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xiao-Dong WenPing Li
Aug 3, 2019·Journal of Veterinary Pharmacology and Therapeutics·Yu LiuYinsheng Qiu
Jun 12, 2014·Biomedical Chromatography : BMC·Yan ZhaoQingguo Wang

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