SPE-UPLC-MS/MS for the determination of phthalate monoesters in rats urine and its application to study the effects of food emulsifier on the bioavailability of priority controlling PAEs

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
R XuW L Ji

Abstract

This research was mainly focused on the effects of food emulsifier on the bioavailability of six priority controlling phthalate acid esters (PAEs) for the further accurate assessment of their toxic effects, using the corresponding phthalate acid monoesters (PAMEs) in rats urine as biomarkers. Glycerin monostearate was chosen as typical food emulsifier. A method was established to determine PAMEs in urine from rats either in experimental group (integrated gavaged with glycerin monostearate and PAEs) or in control group (gavaged with PAEs only), by using solid-phase extraction (SPE) coupled with ultra performance liquid chromatography tandem mass spectrometry (SPE-UPLC-MS/MS). Extraction recoveries were more than 75% for all the PAMEs; the calibration curve was linear in the range of 1.0-1000.0ng/mL with R(2)>0.995; the limits of detection (LOD) were 0.30ng/mL-0.50ng/mL. In addition, by analysing quality control (QC) urine samples in 3 days, it showed that the method was precise and accurate, for the intra-day and inter-day RSD within 16%, and the accuracy more than 82%. Internal exposure amount of all PAEs in experimental group was significantly higher than that in control group with p values of less than 0.05 except for butyl b...Continue Reading

References

Oct 18, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Tatsuhiro NiinoSukeo Onodera
Jan 25, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ralf PreussJürgen Angerer
Oct 9, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Núria MonfortJordi Segura
Aug 26, 2014·Journal of AOAC International·Ji-Cai FanZhong-Qiao Yang

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Citations

Aug 12, 2017·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Zigang TangXuejun Kang

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