Apr 10, 2007

Specialized techniques for site-directed mutagenesis in cyanobacteria

Methods in Molecular Biology
Eugenia M ClericoSusan S Golden

Abstract

Synechococcus elongatus PCC 7942 is an excellent model system for studying the molecular mechanism of the circadian clock in cyanobacteria. The "plastic" genetic characteristics of this organism have facilitated the development of various methods for mutagenesis of its chromosome. These methods are based on homologous recombination between the chromosome and foreign DNA, introduced to the cyanobacteria by either transformation or conjugation. Here we describe different approaches to mutagenize the chromosome of S. elongatus, including insertional mutagenesis, hit-and-run allele replacement, rps12-mediated gene replacement, and regulated expression of genes from ectopic sites, the neutral sites of the S. elongatus genome.

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Mentioned in this Paper

Streblus elongatus
Synechococcus
Transformation, Genetic
Conjugation
Alkalescens-Dispar Group
Circadian Clocks
Mutagenesis, Site-Directed
Recombination, Genetic
MRPS12 gene
RPS12 gene

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