Specialized transcription factories.

Biochemical Society Symposium
Jon BartlettPeter R Cook

Abstract

We have previously suggested a model for the eukaryotic genome based on the structure of the bacterial nucleoid where active RNA polymerases cluster to loop the intervening DNA. This organization of polymerases into clusters--which we call transcription 'factories'--has important consequences. For example, in the nucleus of a HeLa cell the concentration of soluble RNA polymerase II is approximately 1 mM, but the local concentration in a factory is 1000-fold higher. Because a promoter can diffuse approximately 100 nm in 15 s, one lying near a factory is likely to initiate; moreover, when released at termination, it will still lie near a factory, and the movement and modifications (e.g. acetylation) accompanying elongation will leave it in an 'open' conformation. Another promoter out in a long loop is less likely to initiate, because the promoter concentration falls off with the cube of the distance from the factory. Moreover, a long tether will buffer it from transcription-induced movement, making it prone to deacetylation, deposition of HP1 (heterochromatin protein 1), and incorporation into heterochromatin. The context around a promoter will then be self-sustaining: productive collisions of an active promoter with the factory ...Continue Reading

Citations

May 21, 2008·The Journal of Cell Biology·Meng Xu, Peter R Cook
Jul 18, 2008·Biochemical Society Transactions·David R F CarterPeter R Cook
Aug 12, 2009·Nature Reviews. Genetics·Peggy J Farnham
Sep 4, 2009·Chromosoma·Dave A Pai, David R Engelke
Nov 17, 2011·The EMBO Journal·Jesse R RaabRohinton T Kamakaka
Mar 27, 2007·Cold Spring Harbor Symposia on Quantitative Biology·M J DyeN J Proudfoot
Sep 11, 2009·Nature·Anita Göndör, Rolf Ohlsson
Oct 19, 2010·Plant Molecular Biology·Tatiana V KomarovaYuri L Dorokhov
Nov 1, 2006·Biochemical Society Transactions·I Faro-Trindade, P R Cook
Jun 1, 2013·Biomolecular Concepts·Justin M O'SullivanAusten R D Ganley
Apr 17, 2014·Epigenetics : Official Journal of the DNA Methylation Society·Ekaterina S GushchanskayaSergey V Razin
Mar 9, 2011·The Journal of Cell Biology·Indika Rajapakse, Mark Groudine
Feb 15, 2011·Biochemistry. Biokhimii︠a︡·S V RazinO V Yarovaya
Feb 24, 2009·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Christophe Lavelle
Feb 20, 2010·PLoS Computational Biology·Ivan JunierFrançois Képès
May 18, 2007·Annual Review of Genomics and Human Genetics·Douglas R HiggsRichard Gibbons
Jan 25, 2007·ACS Chemical Biology·Anna K Mapp, Aseem Z Ansari
Apr 20, 2013·Chemical Reviews·Argyris Papantonis, Peter R Cook
Feb 7, 2007·Trends in Genetics : TIG·Davide MarenduzzoPeter R Cook
Apr 21, 2007·Trends in Genetics : TIG·Florian M PaulerDenise P Barlow
Oct 2, 2007·Progress in Retinal and Eye Research·Ales Cvekl, Melinda K Duncan
Aug 19, 2008·Biochimica Et Biophysica Acta·Meng Xu, Peter R Cook
Dec 7, 2007·Genes & Development·Robert Schneider, Rudolf Grosschedl
Jan 31, 2012·Seminars in Cancer Biology·Binwei DengPeter R Cook

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