Species Dependence of SYTO 9 Staining of Bacteria

Frontiers in Microbiology
Cushla McGoverinFrédérique Vanholsbeeck


SYTO 9 is a fluorescent nucleic acid stain that is widely used in microbiology, particularly for fluorescence microscopy and flow cytometry analyzes. Fluorimetry-based analysis, i.e., analysis of fluorescence intensity from a bulk sample measurement, is more cost effective, rapid and accessible than microscopy or flow cytometry but requires application-specific calibration. Here we show the relevance of SYTO 9 for food safety analysis. We stained four bacterial species of relevance to food safety (Bacillus cereus, Escherichia coli, Salmonella enterica subspecies enterica ser. Typhimurium, Staphylococcus aureus) with different concentrations of SYTO 9, with and without the presence of ethylenediaminetetraacetic acid (EDTA), for varying amounts of time, to investigate the effect of these treatment parameters on fluorescence intensity. The addition of EDTA and an increased staining duration did not significantly affect fluorescence intensity, and over the bacterial cell concentration range investigated (∼105-108 CFU/ml) there was no significant difference in using 0.5 or 1 μM SYTO 9. The effect of bacterial cell concentration on fluorescence intensity was species specific. At different bacterial cell concentrations, the effect of ...Continue Reading


Nov 22, 1965·Biochemical and Biophysical Research Communications·L Leive
Jul 2, 1998·Applied and Environmental Microbiology·D J MasonV A Gant
Aug 4, 2004·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Janne LehtinenEsa-Matti Lilius
Sep 4, 2007·Biophysical Journal·Galina ReshesMario Feingold
Nov 8, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Irene O L McHugh, Ayly Ling Tucker
Oct 18, 2008·Journal of Microbiological Methods·Alice PascaudGuy Soulas
Sep 3, 2009·Journal of Food Protection·Byron Brehm-StecherMary Lou Tortorello
Nov 7, 2009·Applied Spectroscopy·Miryeong SohnPaula J Fedorka-Cray
Apr 3, 2012·Molecular and Cellular Probes·Mar LarrosaMaría Teresa García-Conesa
Apr 17, 2012·Environmental Science & Technology·Belinda M HewittSimon Swift
Jul 19, 2012·World Journal of Microbiology & Biotechnology·T ZottaA Ricciardi
Apr 5, 2013·Clinical Microbiology Reviews·Anna Fàbrega, Jordi Vila
Jul 10, 2017·Analytical and Bioanalytical Chemistry·Wendy DonosoLeonardo S Santos

Related Concepts

Bacillus cereus
Edetic Acid
Escherichia coli
Flow Cytometry
Microscopy, Fluorescence
Nucleic Acids
Salmonella typhimurium

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Systemic Juvenile Idiopathic Arthritis

Systemic juvenile idiopathic arthritis is a rare rheumatic disease that affects children. Symptoms include joint pain, but also fevers and skin rashes. Here is the latest on this disease.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Pontocerebellar Hypoplasia

Pontocerebellar hypoplasias are a group of neurodegenerative autosomal recessive disorders with prenatal onset, atrophy or hypoplasia of the cerebellum, hypoplasia of the ventral pons, microcephaly, variable neocortical atrophy and severe mental and motor impairments. Here is the latest research on pontocerebellar hypoplasia.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.

Chronic Traumatic Encephalopathy

Chronic Traumatic Encephalopathy (CTE) is a progressive degenerative disease that occurs in individuals that suffer repetitive brain trauma. Discover the latest research on traumatic encephalopathy here.

Related Papers

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
Donald WlodkowicZbigniew Darzynkiewicz
Cytometry. Part a : the Journal of the International Society for Analytical Cytology
Anirudh J UllalCharles F Reich
© 2021 Meta ULC. All rights reserved