PMID: 9172049Jun 1, 1997Paper

Species differences in PAF receptor binding in the lungs between hamster and guinea pig

Journal of Lipid Mediators and Cell Signalling
J Chen, N Giris

Abstract

Platelet-activating factor (PAF) receptor in normal Golden Syrian hamster lung was characterized using radioligand binding studies and compared with guinea pig lung PAF receptor. [3H]WEB2086, a potent and specific PAF antagonist, was used as a radioligand for equilibrium binding, kinetic studies, competitive binding in receptor preparation (0-110000 g fraction of lung homogenate) from hamster and guinea pig lungs. Binding of [3H]WEB 2086 to the receptor preparation was saturable, reversible and specific in both hamster and guinea pig lungs. Scatchard plot analysis of equilibrium binding data indicates a single binding site in hamster lung with the equilibrium dissociation constant (KD) of 66.1 +/- 36.7 nM (n = 4) and maximal binding (Bmax) of 135.4 +/- 63.1 fmol/mg, but two binding sites in guinea pig lung with a high affinity site (KD = 1.7 +/- 0.6 nM; Bmax = 48.6 +/- 2.6 fmol/mg) and a low affinity site (KD = 83.8 +/- 32 nM; Bmax = 480.8 +/- 158 fmol/mg). The heterogeneity of [3H]WEB2086 binding to guinea pig lung but not to hamster lung was also confirmed by dissociation kinetic studies, in which biphasic dissociation kinetic was shown in guinea pig and monophasic kinetic in hamster lung. Although the specific [3H]WEB 2086 b...Continue Reading

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