Species identification of blood and bloodstains by enzyme-linked immunosorbent assay (ELISA) using anti-human immunoglobulin kappa light chain monoclonal antibody

Forensic Science International : Synergy
Y YamamotoHideo Ishizu

Abstract

A series of experiments was conducted to establish a species identification method by means of the enzyme-linked immunosorbent assay (ELISA) using monoclonal antibody with high specificity, the result of which is reported. The limit of human IgG detection by inhibition ELISA using mouse anti-human immunoglobulin kappa light chain (Ig kappa) monoclonal antibody is 0.01 microgram. Using this method it was possible to detect human Ig kappa from bloodstains in dilutions up to 1:100,000 (dilution multiple of bloodstain extract to the original amount of blood). Human Ig kappa was detectable in human bloodstains in dilutions up to 1:50,000 one year after stain preparation and in dilutions up to 1:10,000 4 years after stain preparation, showing that this method is applicable to species identification of old bloodstains. When primates other than human were studied for specificity, red-chested tamarin and Japanese monkey showed no cross reaction at all. Cross reaction was noted with a chimpanzee, but experimentally it could be differentiated from human. Furthermore, no cross reaction was noted with respect to blood samples from non-primates (guinea pig, sheep, horse, dog and cat).

References

Jan 1, 1986·Zeitschrift für Rechtsmedizin. Journal of legal medicine·H TsutsumiY Katsumata
Oct 1, 1983·Journal - Forensic Science Society·R W Butt

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Citations

Jul 7, 2009·Forensic Science International : Synergy·Ian P HurleyJohn H H Williams
Nov 26, 2016·International Journal of Legal Medicine·Elina ZailerYulia B Monakhova

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