PMID: 8590311Nov 1, 1995Paper

Species-specific activity of rat recombinant interleukin-1 beta

Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research
C LiuR P Hart

Abstract

We have constructed expression clones for mature rat interleukin-1 beta (IL-1 beta). Using these clones, rat recombinant IL-1 beta (rrIL-1 beta) was prepared and purified by a single-step cation-exchange chromatography method. In addition, synthetic rrIL-1 beta mRNA was transcribed and used to synthesize radiolabeled 35S-rrIL-1 beta for binding studies. The rrIL-1 beta produced was demonstrated to have IL-1 bioactivity in a mouse thymocyte proliferation assay, in which it could be blocked by inclusion of human recombinant IL-1 receptor antagonist (hrIL-1Ra). Similarly, rrIL-1 beta induced LIF mRNA expression in cultured rat sympathetic ganglia. In each case, the maximal dose of rrIL-1 beta was similar to that of human IL-1 beta. However, the peak response in rat tissue was twofold higher with rat IL-1 beta than with human IL-1 beta. The expression of rrIL-1 beta provides a useful tool for studying IL-1 receptors in rat, in which human and mouse IL-1 beta have been found to bind poorly. Species-specific activity of rat IL-1 beta may correlate with compensatory ionic properties of rat IL-1 receptor.

References

Jul 1, 1991·Journal of Neuroscience Research·R P HartG M Jonakait
Jan 1, 1991·Brain Research. Molecular Brain Research·G A Higgins, J A Olschowka
Aug 1, 1986·The Journal of Experimental Medicine·D GiulianL B Lachman
Jul 1, 1972·The Journal of Experimental Medicine·I GeryB H Waksman
Sep 1, 1984·Journal of Leukocyte Biology·R F Kampschmidt
Jan 1, 1995·Endocrinology·K SchotanusF J Tilders
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·S M MolineauxT T Yamin
Feb 1, 1986·Immunology Today·J J OppenheimS K Durum

❮ Previous
Next ❯

Citations

Jul 12, 2002·Journal of Neuroscience Research·Jonathan Z PanRonald P Hart
Dec 10, 1998·Toxicology·R J VandebrielC Meredith
May 28, 1996·Proceedings of the National Academy of Sciences of the United States of America·C M TroyM L Shelanski
Feb 13, 2003·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Patric M SchiltzCarol A Kruse
Oct 22, 1998·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·P TaishiJ M Krueger
Dec 10, 1998·Journal of Neuroimmunology·Y Bai, R P Hart

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Toxicology in Vitro : an International Journal Published in Association with BIBRA
C Meredith, K Miller
Pediatric Nephrology : Journal of the International Pediatric Nephrology Association
Fangming Lin
© 2021 Meta ULC. All rights reserved