Species-specific and ubiquitous DNA-based assays for rapid identification of Staphylococcus epidermidis.

Journal of Clinical Microbiology
F MartineauM G Bergeron

Abstract

Staphylococcus epidermidis is an aerobic gram-positive coccus that is now recognized among the coagulase-negative staphylococci as an etiological agent with an important range of pathogenicity in humans. Several diagnostic kits based on biochemical or immunological reactions can efficiently identify Staphylococcus aureus. However, these tests are often unreliable for the identification of coagulase-negative staphylococcal species including S. epidermidis. Since DNA-based assays for the species-specific identification of S. epidermidis remain unavailable, we have developed such tests in order to improve the accuracy and the rapidity of tests for the diagnosis of S. epidermidis infections. On the basis of the results of hybridization assays with clones randomly selected from an S. epidermidis genomic library, we identified a chromosomal DNA fragment which is specific and 100% ubiquitous for the identification of S. epidermidis. This 705-bp fragment was sequenced and used to design PCR amplification primers. PCR assays with the selected primers were also highly specific and ubiquitous for the identification from bacterial cultures of clinical isolates of S. epidermidis from a variety of anatomic sites. While three strains of S. ca...Continue Reading

References

Jan 1, 1975·Journal of Clinical Microbiology·W E Kloos, K H Schleifer
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Jul 1, 1992·Clinical Microbiology Reviews·C E Stager, J R Davis
Jul 1, 1991·Journal of Medical Microbiology·J A Lindsay, T V Riley
Jan 1, 1989·Annals of Internal Medicine·M A MartinR P Wenzel
Jul 1, 1988·Clinical Microbiology Reviews·M A Pfaller, L A Herwaldt
Mar 1, 1987·Infection Control : IC·R I StillmanL C Donowitz
Jan 1, 1986·American Journal of Clinical Pathology·P M Leighton, J A Little
Sep 1, 1982·Journal of Clinical Microbiology·W E Kloos, J F Wolfshohl
Aug 1, 1982·Journal of Clinical Microbiology·C M SewellR K Guthrie
Aug 1, 1994·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·M E Rupp, G L Archer
Aug 1, 1994·Journal of Clinical Microbiology·L StoakesZ Hussain
Jan 1, 1994·Diagnostic Microbiology and Infectious Disease·C E GrantJ A Williams
Jan 1, 1994·Clinical Microbiology Reviews·W E Kloos, T L Bannerman

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Citations

Feb 8, 2008·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Ana Lúcia Souza AntunesPedro Alves D'Azevedo
Feb 20, 2013·Journal of Peptide Science : an Official Publication of the European Peptide Society·C AvitabileA Romanelli
Jul 24, 2007·European Journal of Orthopaedic Surgery & Traumatology : Orthopédie Traumatologie·Fergus J ByrneMichael Kerin
Oct 9, 2015·Journal of Medical Microbiology·Claire L McMurrayPeter M Hawkey
Mar 23, 2004·FEMS Microbiology Letters·Monika Ehling-SchulzSiegfried Scherer
Jun 11, 2005·Extremophiles : Life Under Extreme Conditions·Lemese Ah Tow, Don A Cowan
Nov 29, 2007·The ISME Journal·Issmat I KassemMalak A Esseili
Feb 5, 2011·Microbiology·Sarah E RoweJames P O'Gara
Mar 3, 2012·Journal of Applied Microbiology·F K TavariaM E Pintado
Oct 22, 2013·Folia Microbiologica·Mária MikulášováAnna Belicová
Dec 15, 2015·BMC Microbiology·Hend ZeitounNourhan Fanaki
Feb 11, 2005·Folia Microbiologica·F RůzickaV Woznicová
Sep 3, 2005·Molecular Diagnosis : a Journal Devoted to the Understanding of Human Disease Through the Clinical Application of Molecular Biology·Farjana B RowtherVinay R Joshi
Feb 27, 2008·Transfusion·Melanie StörmerJens Dreier
Dec 14, 2011·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Odelta Dos SantosPedro Alves D'Azevedo
Aug 31, 2004·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M L MorettiD Blum-Menezes
Dec 21, 2010·Applied and Environmental Microbiology·Elrike FrenzelMonika Ehling-Schulz
Oct 31, 2017·Asian Pacific Journal of Tropical Medicine·Henok A TegegneRenata Karpíšková
Sep 5, 2019·International Forum of Allergy & Rhinology·Brett Wagner MackenzieKristi Biswas
Mar 17, 2018·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Xiqi LiSamuel A Shelburne
Apr 5, 2021·Journal of Applied Microbiology·A G Al-BakriK Becker
Oct 6, 2000·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·D R KeechP Mathers
May 11, 2021·ACS Nano·Mohamed A Abdou MohamedWarren C W Chan

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