Species-Specific Detection of Vibrio anguillarum in Marine Aquaculture Environments by Selective Culture and DNA Hybridization.

Applied and Environmental Microbiology
J Martínez-PicadoJ Jofre

Abstract

Methods for specific detection of Vibrio anguillarum in complex microbial communities within diverse marine aquaculture environments were evaluated. A system for the detection of culturable cells based on the combined use of a selective medium and a nonradioactively labeled oligodeoxynucleotide complementary to 16S rRNA was developed. Four hundred fourteen bacterial cultures were evaluated in order to assess the specificity of the method. When both the selective medium and the specific probe gave positive results, the cultures were always identified as V. anguillarum. The selectivity for colony hybridization was 1 V. anguillarum cell in 10,000 total bacterial cells in environmental samples. The utility of the method was also compared with detection by dot blot hybridization of either raw DNA purified from environmental samples or PCR-amplified DNA of 16S rRNA genes, using universal eubacterial primers. The post-PCR hybridization was more sensitive (8 x 10(sup2) cells) than direct hybridization of the whole purified DNA (10(sup6) cells). However, the selective medium-probe combined method was as sensitive as post-PCR hybridization, albeit more specific.

References

Oct 1, 1992·Applied and Environmental Microbiology·A L ReysenbachN R Pace
Jan 1, 1991·Annual Review of Microbiology·R J Steffan, R M Atlas
Jan 1, 1991·The Journal of Applied Bacteriology·J M Huguet, F Ribas
Mar 1, 1991·Applied and Environmental Microbiology·W E HillK A Lampel
Jan 1, 1987·Methods in Enzymology·D M Wallace
Mar 1, 1989·Applied and Environmental Microbiology·C C SomervilleR R Colwell
Mar 1, 1986·Applied and Environmental Microbiology·U B Sørensen, J L Larsen
May 1, 1994·Applied and Environmental Microbiology·M AlsinaA R Blanch
Jan 1, 1994·The Journal of Applied Bacteriology·M Alsina, A R Blanch
Feb 1, 1993·Applied and Environmental Microbiology·W H KochT A Cebula
Jan 1, 1955·Acta Pathologica Et Microbiologica Scandinavica·V MØLLER

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Citations

Sep 9, 2004·Microbiology and Molecular Biology Reviews : MMBR·Fabiano L ThompsonJean Swings
Feb 12, 2004·Environmental Microbiology·Marta Cerdà-Cuéllar, Anicet R Blanch
Feb 6, 2008·Journal of Applied Microbiology·I Noguerola, A R Blanch
Feb 2, 2000·Applied and Environmental Microbiology·M Cerdà-CuéllarA R Blanch
May 24, 2019·Analytical Chemistry·Lide GuJuewen Liu

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