PMID: 490629Aug 1, 1979Paper

Species-specific effects on the optical signals of voltage-sensitive dyes

The Journal of Membrane Biology
W N Ross, L F Reichardt

Abstract

The absorption changes of two merocyanine dyes in response to membrane potential changes were measured on several neuronal preparations to see whether the dyes would be useful in recording from these cells. We were able to record large signals without averaging from barnacle and leech neurons. The greatest signal with WW375 was seen at 750 nm. Much smaller increases in transmitted light intensity were seen at all other wavelengths between 500 and 780 nm. In contrast, vertebrate neuronal preparations produced much smaller signals with an entirely different action spectrum. Essentially the same spectrum was seen in cells of the sympathetic ganglion of the bullfrog, Rana catesbiana, dissociated chick spinal cord neurons, or dissociated rat superior cervical ganglion neurons. In each case an action potential was accompanied by increases in transmitted light intensity between 500 and 600 nm and 730 and 780 nm, and decreases in intensity between 600 and 730 nm with the dye WW375, the best dye tested. Similar results were obtained with dye NK2367 on both vertebrate and invertebrate preparations, except that the spectral properties were shifted 30 nm towards the blue. Both dyes caused some photodynamic damage to the cultured neurons af...Continue Reading

References

Jan 1, 1978·Reviews of Physiology, Biochemistry and Pharmacology·L B Cohen, B M Salzberg
Jan 1, 1975·Annual Review of Biophysics and Bioengineering·F Conti
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·P H O'LagueD D Potter
Jan 1, 1974·The Journal of Membrane Biology·L B CohenC H Wang
Oct 1, 1972·The Journal of General Physiology·J Pooler
Aug 1, 1969·The Journal of Physiology·D A Baylor, J G Nicholls
Jun 1, 1970·The Journal of Physiology·H M BrownR M Meech
Jul 1, 1963·The Journal of Physiology·J G BLACKMANC RAY

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Citations

Feb 15, 1981·The Journal of Membrane Biology·R K GuptaC H Wang
Aug 27, 2013·The Journal of Membrane Biology·Saad Habib-E-Rasul MullahKatsushige Sato
Aug 1, 1995·Progress in Neurobiology·T J Ebner, G Chen
Dec 22, 2000·Progress in Neurobiology·Y Momose-SatoK Kamino
Oct 22, 2004·Nature Reviews. Neuroscience·Amiram Grinvald, Rina Hildesheim
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·A GrinvaldI Farber
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·V Lev-Ram, A Grinvald
Dec 1, 1980·The Journal of General Physiology·S Nakajima, A Gilai
Jan 7, 2009·Journal of Biomedical Optics·Joseph Foley, Martin Muschol
Apr 11, 2001·The Japanese Journal of Physiology·T Sakai, K Kamino
Nov 16, 2005·The Japanese Journal of Physiology·Tetsuro Sakai
Feb 14, 1998·IEEE Engineering in Medicine and Biology Magazine : the Quarterly Magazine of the Engineering in Medicine & Biology Society·R T Bove, S M Dillon
Apr 18, 2006·Autonomic Neuroscience : Basic & Clinical·Yoko Momose-Sato, Katsushige Sato
Sep 18, 2014·Biophysical Journal·Jeremy S TregerFrancisco Bezanilla
Jan 6, 2016·Cold Spring Harbor Protocols·Amiram GrinvaldRina Hildesheim

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