PMID: 25739282Mar 6, 2015Paper

Species-specific localization of DNA from pericentromeric heterochromatin on polytene chromosomes in the salivary gland cells and 3D-nuclear organization nurse cells in Drosophila virilis, and Drosophila kanekoi (Diptera: Drosophilidae)

Genetika
K E UsovV N Stegniĭ

Abstract

Microdissection of the chromocenter of D. virilis salivary gland polytene chromosomes has been carried out and the region-specific. DNA library (DvirIII) has been obtained. FISH was used for DvirIII hybridization with salivary gland polytene chromosomes and ovarian nurse cells of D. virilis and D. kanekoi. Localization of DvirIII in the pericentromeric regions of chromosomes and in the telomeric region of chromosome 5 was observed in both species. Moreover, species specificity in the localization of DNA sequences of DvirIII in some chromosomal regions was detected. In order to study the three-dimensional organization of pericentromeric heterochromatin region of polytene chromosomes of ovarian nurse cells of D. virilis and D. kanekoi, 3D FISH DvirIII was performed with nurse cells of these species. As a result, species specificity in the distribution of DvirIII signals in the nuclear space was revealed. Namely, the signal was detected in the local chromocenter at one pole of the nucleus in D. virilis, while the signal from the telomeric region of chromosome 5 was detected on another pole. At the same time, DvirIII signals in D. kanekoi are localized in two separate areas in the nucleus: the first belongs to the pericentromeric r...Continue Reading

References

Jan 1, 1986·The Journal of Cell Biology·M HochstrasserJ W Sedat
Jan 1, 1985·Annals of the New York Academy of Sciences·L Manuelidis
Jan 1, 1980·Annual Review of Genetics·D L Brutlag
Dec 7, 2007·Genes & Development·Robert Schneider, Rudolf Grosschedl

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Citations

Nov 19, 2014·PloS One·Roberta MoschettiPatrizio Dimitri
Nov 19, 2004·International Review of Cytology·I F ZhimulevE N Andreyeva

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