PMID: 9431963Feb 12, 1998Paper

Species-specific plasmid sequences for PCR identification of the three species of Borrelia burgdorferi sensu lato involved in Lyme disease

Journal of Clinical Microbiology
M C Misonne, P P Hoet

Abstract

Species-specific sequences were shown to be carried by plasmids of the three main species of Borrelia burgdorferi sensu lato involved in Lyme disease. Libraries of the 16-, 33-, and 25-kb plasmids of B. burgdorferi sensu stricto, Borrelia garinii, and Borrelia afzelii, respectively, were then built and used to isolate species-specific sequences. After sequencing of the cloned inserts, three sets of primers were designed. They were shown to determine species-specific PCR amplification products. The sensitivities of the PCR assay with these primers were 100 spirochetes for B. burgdorferi sensu stricto and 1,000 spirochetes for B. garinii and B. afzelii. The usefulness of these primers for the identification of species in biological samples (tick, serum, and cerebrospinal fluid samples) was ascertained.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jul 1, 1992·International Journal of Systematic Bacteriology·G BarantonP A Grimont
Dec 1, 1992·The American Journal of Tropical Medicine and Hygiene·B J JohnsonJ Piesman
Mar 1, 1990·Journal of Clinical Microbiology·D H PersingS W Barthold
Mar 1, 1991·Journal of Clinical Microbiology·P A RosaT G Schwan
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Mar 1, 1988·Journal of Clinical Microbiology·A G Barbour
Jan 1, 1993·Microbiology and Immunology·H KawabataY Yanagihara
Dec 1, 1993·Journal of Clinical Microbiology·B C ZinggR B LeFebvre
Jul 1, 1993·Research in Microbiology·D PosticG Baranton
Apr 1, 1993·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M V AssousG Baranton
Oct 1, 1995·Journal of Clinical Microbiology·Y Xu, R C Johnson
Mar 1, 1996·Infection and Immunity·E IsogaiH Isogai

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Citations

Jan 4, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Kathryn L JonesAllen C Steere

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