Species-Specific Recognition of Sulfolobales Mediated by UV-Inducible Pili and S-Layer Glycosylation Patterns.

MBio
Marleen van WolferenSonja-Verena Albers

Abstract

The UV-inducible pili system of Sulfolobales (Ups) mediates the formation of species-specific cellular aggregates. Within these aggregates, cells exchange DNA to repair DNA double-strand breaks via homologous recombination. Substitution of the Sulfolobus acidocaldarius pilin subunits UpsA and UpsB with their homologs from Sulfolobus tokodaii showed that these subunits facilitate species-specific aggregation. A region of low conservation within the UpsA homologs is primarily important for this specificity. Aggregation assays in the presence of different sugars showed the importance of N-glycosylation in the recognition process. In addition, the N-glycan decorating the S-layer of S. tokodaii is different from the one of S. acidocaldarius Therefore, each Sulfolobus species seems to have developed a unique UpsA binding pocket and unique N-glycan composition to ensure aggregation and, consequently, also DNA exchange with cells from only the same species, which is essential for DNA repair by homologous recombination.IMPORTANCE Type IV pili can be found on the cell surface of many archaea and bacteria where they play important roles in different processes. The UV-inducible pili system of Sulfolobales (Ups) pili from the crenarchaeal S...Continue Reading

References

Dec 1, 1992·Canadian Journal of Microbiology·C L MasyM M Mestdagh
Dec 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·H C Winther-LarsenM Koomey
Apr 22, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yinuo LiWenyuan Shi
Aug 22, 2003·Protein Science : a Publication of the Protein Society·Sonia L BardyKen F Jarrell
Oct 26, 2005·FEMS Microbiology Letters·Norio Kurosawa, Dennis W Grogan
Oct 2, 2007·Journal of Bacteriology·Sabrina FrölsChrista Schleper
Oct 13, 2007·Genome Biology·Dorothee GötzMalcolm F White
Jul 16, 2008·Journal of Bacteriology·Sandy Y M NgKen F Jarrell
Apr 7, 2009·Extremophiles : Life Under Extreme Conditions·Sonja-Verena Albers, Mecky Pohlschröder
May 12, 2009·Journal of Bacteriology·Martin ClausenBerenike Maier
Oct 27, 2009·Journal of Bacteriology·Behnam ZolghadrSonja-Verena Albers
Apr 7, 2010·Journal of Bacteriology·Manuela TripepiMechthild Pohlschröder
Apr 7, 2010·Journal of Bacteriology·Michelle Qiu CarterStephen Lory
Dec 1, 2010·Journal of Applied Microbiology·E V Soares
Mar 18, 2011·FEMS Microbiology Letters·Ken F JarrellJames P J Chong
May 17, 2011·Nature Reviews. Microbiology·Sonja-Verena Albers, Benjamin H Meyer
Oct 14, 2011·Molecular Microbiology·Thorsten Allers
Oct 18, 2011·Molecular Microbiology·Małgorzata AjonChrista Schleper
Mar 1, 2012·PLoS Biology·Hinsby Cadillo-QuirozRachel J Whitaker
Mar 1, 2012·Virulence·Mathieu CoureuilXavier Nassif
May 23, 2012·Trends in Microbiology·Ken F Jarrell, Sonja-Verena Albers
Jun 19, 2012·Frontiers in Microbiology·Michaela WagnerSonja-Verena Albers
Oct 20, 2012·Environmental Microbiology·Anna-Lena HencheSonja-Verena Albers
Dec 4, 2012·Microbiology and Molecular Biology Reviews : MMBR·Carmen L GiltnerLori L Burrows
Jan 30, 2013·Biochemical Society Transactions·Lina KaminskiJerry Eichler
Apr 17, 2013·Journal of Proteome Research·Gianna PalmieriGabriella Pocsfalvi
Jun 25, 2013·Journal of Bacteriology·Rianne N EsquivelMechthild Pohlschroder
Oct 10, 2013·MicrobiologyOpen·Marleen van WolferenSonja-Verena Albers
Jan 1, 2013·Biology·Miao-Chiu Hung, Myron Christodoulides
Jun 2, 2014·Nature Medicine·Sandra C BernardSandrine Bourdoulous
Oct 27, 2015·Trends in Microbiology·Berenike Maier, Gerard C L Wong
Feb 18, 2016·Proceedings of the National Academy of Sciences of the United States of America·Marleen van WolferenSonja-Verena Albers
May 21, 2016·Frontiers in Microbiology·Kira S MakarovaSonja-Verena Albers
Oct 22, 2016·Trends in Microbiology·Hanne L P Tytgat, Willem M de Vos
Oct 5, 2016·Nature Communications·Subramania KolappanLisa Craig
May 16, 2017·Nature Reviews. Microbiology·Alexander WagnerSonja-Verena Albers
Jun 7, 2017·Analytical and Bioanalytical Chemistry·Asif ShajahanParastoo Azadi
Jul 21, 2017·Frontiers in Microbiology·Yarden ShalevUri Gophna

❮ Previous
Next ❯

Citations

Sep 26, 2020·Biochemical and Biophysical Research Communications·James A Shapiro

❮ Previous
Next ❯

Methods Mentioned

BETA
DNA exchange
electron microscopy
fluorescence microscopy
glycosylation
PCR
transmission electron microscopy

Software Mentioned

ImageJ
ClustalW
GenScript
Byonic

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.