PMID: 9531493Jun 11, 1998Paper

Species-specificity in endoplasmic reticulum signal peptide utilization revealed by proteins from Trypanosoma brucei and Leishmania

The Biochemical Journal
A Al-QahtaniKojo Mensa-Wilmot

Abstract

N-Terminal signal peptides direct secretory and most membrane proteins into the exocytic pathway at the endoplasmic reticulum. Signal sequences can function across kingdoms. However, our attempts at translocating variant surface glycoprotein (VSG) 117, VSG MVAT7, VSG 221 and BiP from Trypanosoma brucei and gp63 from Leishmania chagasi into canine pancreas microsomes failed. On replacing the signal peptide of VSG 117 with that from yeast prepro-alpha-mating factor (ppalphaMF) the chimaeric protein was imported, indicating that the signal sequence of VSG 117 was incompatible with the protein-import machinery of mammalian microsomes. Replacement of the gp63-h-region with a hybrid composed of the N-terminal nine residues from the h-region of gp67 from Autographa californica nuclear polyhedrosis virus and the C-terminal 10 residues from the h-region of gp63 from L. major produced a functional signal peptide. Thus, the h-region of kinetoplastid signal peptides appears to be the subdomain that is non-functional at the mammalian translocon. The calculated biophysical properties and computed discriminant scores (predictive of importability of signal peptides into mammalian microsomes) of the kinetoplastid signal sequences nevertheless a...Continue Reading

Citations

Apr 28, 2004·Molecular and Biochemical Parasitology·Chaoqun YaoMary E Wilson
Dec 19, 2009·The Biochemical Journal·Josh DuffyKojo Mensa-Wilmot
Jan 7, 2018·Cellular and Molecular Life Sciences : CMLS·Victor Van Puyenbroeck, Kurt Vermeire
Jun 21, 2008·Parasitology Research·Marcel Marín-VillaYara M Traub-Cseko
Dec 14, 1999·The Journal of Biological Chemistry·T Zheng, C V Nicchitta
Feb 7, 2001·The Journal of Biological Chemistry·P W DennyD F Smith
Oct 13, 2006·Molecular and Cellular Biology·Nirit Mor-VakninDavid M Markovitz
Mar 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Malcolm J McConvilleRohan D Teasdale
Nov 7, 2000·Parasitology Today·T Ilg
Oct 18, 2003·Molecular and Biochemical Parasitology·Chaoqun YaoMary E Wilson

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