Species specificity of anticoagulant activity of activated human protein C: involvement of factor V as well as protein S

Thrombosis Research
Y KatsuuraS Miyamoto

Abstract

Activated protein C (APC) possesses species specificity in its anticoagulant activity. Human APC exerts only weak activity in rat plasma compared with that in human plasma. The present study was undertaken to estimate the difference in interaction of human and rat factors with human APC and to assess the cause of the species specificity. Human or rat protein S (PS), factor V, or factor VIII was used to supplement human plasma depleted of each respective factor, and the anticoagulant activity of human APC was measured in term of the elongation of activated partial thromboplastin time (APTT). The activity of human APC in rat PS- or factor V-supplemented plasma was weaker than that in the human PS- or factor V-supplemented plasma. Furthermore, using purified human and rat factor V, human APC showed weaker inactivation of rat factor V than human factor V. Equal anticoagulant activity was observed in human or rat factor VIII-supplemented plasma. And there was a little difference in the interaction of APC with its inhibitors in human or rat plasma during a few minutes of incubation as judged by measurement of residual activity by an enzyme capture assay. From these results factor V as well as PS seems to play a major role in the spec...Continue Reading

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Citations

Jun 24, 1998·The Journal of Clinical Investigation·S R CaiK P Ponder
Oct 30, 2008·Thrombosis Journal·Karl MalmBjörn Dahlbäck
Nov 15, 2006·Critical Care : the Official Journal of the Critical Care Forum·Christian LehmannJürgen Birnbaum
Oct 10, 2009·Haematologica·José A FernándezJohn H Griffin
Aug 2, 2007·Journal of Thrombosis and Haemostasis : JTH·S N TchaikovskiG Tans
Apr 11, 2018·Military Medicine·Thomas OrfeoKathleen E Brummel-Ziedins
Dec 17, 2009·Journal of Thrombosis and Haemostasis : JTH·E Castoldi, J Rosing
Jul 22, 2014·Journal of Thrombosis and Haemostasis : JTH·T MatsumotoM Shima
Nov 16, 2019·Clinica Chimica Acta; International Journal of Clinical Chemistry·Julia OtoPilar Medina

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