PMID: 8972859Dec 1, 1996Paper

Species-specificity of rRNA gene transcription in plants manifested as a switch in RNA polymerase specificity

Nucleic Acids Research
J H Doelling, C S Pikaard

Abstract

Rapid evolution of ribosomal RNA (rRNA) gene promoters often prevents their recognition in a foreign species. Unlike animal systems, we show that foreign plant rRNA gene promoters are recognized in an alien species, but tend to program transcription by a different polymerase. In plants, RNA polymerase I transcripts initiate at a TATATA element (+1 is underlined) important for promoter strength and start-site selection. However, transcripts initiate from +32 following transfection of a tomato promoter into Arabidopsis. The rRNA gene promoter of a more closely related species, Brassica oleracea, programs both +1 and +29 transcription. A point mutation at +2 improving the identity between the Brassica and Arabidopsis promoters increases +1 transcription, indicating a role for the initiator element in species-specificity. Brassica +29 transcripts can be translated to express a luciferase reporter gene, implicating RNA polymerase II. TATA mutations that disrupt TATA-binding protein (TBP) interactions inhibit +29 transcription and luciferase expression. Co-expressed TBP proteins bearing compensatory mutations restore +29 transcription and luciferase activity, suggesting a direct TBP-TATA interaction. Importantly, +1 transcription is ...Continue Reading

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Citations

Dec 18, 2004·Plant Molecular Biology·Nataliya Y KomarovaRoman A Volkov
Jan 10, 2002·Biochimica Et Biophysica Acta·E D AkhunovV A Vakhitov
Feb 24, 2000·Nucleic Acids Research·M R Paule, R J White
Jul 21, 2012·Biochemistry. Biokhimii︠a︡·T V KomarovaYu L Dorokhov
Oct 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·J Saez-Vasquez, C S Pikaard
Dec 30, 2015·The Plant Journal : for Cell and Molecular Biology·Roman MatyášekAleš Kovařík
Jun 9, 2012·Molecular and Biochemical Parasitology·Bernardo FrancoImelda López-Villaseñor
Mar 27, 2003·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Lynne Van HerwerdenDavid Blair
Jul 1, 2016·Annual Review of Phytopathology·Andrew O Jackson, Zhenghe Li
Aug 20, 2019·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Oleg GeorgievLuchezar Karagyozov
Aug 8, 2021·Current Genetics·Arjuna Rao VallabhaneniHeather Conrad-Webb

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