Species variations in cDNA sequence and exon splicing patterns in the extensible I-band region of cardiac titin: relation to passive tension

Journal of Muscle Research and Cell Motility
M L GreaserP Mozdziak

Abstract

Titin is believed to play a major role in passive tension development in cardiac muscle. The cDNA sequence of cardiac titin in the I-band sarcomeric region was determined for several mammalian species. Contiguous sequences of 3749, 12,230, 6602, and 11,850 base pairs have been obtained for the rat N2B, rat N2BA, dog N2B, and dog N2BA isoforms respectively. The length of the PEVK region of the N2B isoform did not correlate with rest tension properties since the only species showing an altered length was the dog that expressed a shorter form. No differences were found between the N2B PEVK lengths in ventricular and atrial muscle. New N2BA splicing pathways in the first tandem Ig region were found in human and dog cardiac muscle. Most of the rat and dog sequences were 85-95% identical with the reported human sequence. However, the N2B unique amino acid sequences of rat and dog were only 51 and 67% identical to human. The rat N2B unique sequence was 526 amino acids in length compared to 572 in human. The difference in length was due to deletion of amino acid segments from six different regions of the N2B unique domain. Patterns of PEVK exon expression were also much different in the dog, human, and rat. Six separate dog N2BA PEVK c...Continue Reading

Citations

Feb 9, 2006·Journal of Muscle Research and Cell Motility·Christiane A Opitz, Wolfgang A Linke
Mar 27, 2010·Journal of Biomedicine & Biotechnology·Wei GuoMarion L Greaser
Jan 14, 2018·Nature Communications·David GigantiJorge Alegre-Cebollada
Oct 21, 2006·Cell and Tissue Research·Carole L Moncman, Francisco H Andrade
Nov 23, 2011·Journal of Cellular Biochemistry·Shijun LiMarion L Greaser
Sep 28, 2013·Journal of Cardiovascular Pharmacology·Martin M LeWinter, Henk L Granzier
Feb 21, 2004·Circulation Research·Henk L Granzier, Siegfried Labeit
Nov 19, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Hideaki FujitaHenk L Granzier
Mar 2, 2005·The Journal of General Physiology·Norio FukudaHenk L Granzier
Feb 27, 2013·Circulation·Martin M LeWinter, Henk L Granzier
Feb 28, 2004·Circulation Research·Christiane A OpitzWolfgang A Linke
Oct 1, 2009·Physiological Reviews·Aikaterini Kontrogianni-KonstantopoulosRobert J Bloch
Jan 12, 2013·Nucleic Acids Research·Shijun LiMarion L Greaser
Feb 26, 2019·G3 : Genes - Genomes - Genetics·Kathleen MuenzenFindley R Finseth
Sep 30, 2004·Mechanisms of Development·Chad M WarrenMarion L Greaser
Apr 5, 2008·Journal of Molecular and Cellular Cardiology·Marion L GreaserRichard L Moss

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