Specific action of sodium dodecyl sulfate on the sex factor of Escherichia coli K-12 Hfr strains.

Journal of Bacteriology
N InuzukaM Tomoeda

Abstract

A specific action of sodium dodecyl sulfate (SDS) on the sex (F) factor in the integrated state of Escherichia coli K-12 Hfr H strain is reported. Growth of Hfr cells in Penassay Broth containing SDS results in the elimination of part or all of the F factor, yielding low and nonfertile variants of defective Hfr type and F(+) cells and also F(-) derivatives. Appearance of such variants was generally observed after the culture reached stationary phase. The frequencies of F(-) cells then increased. F(-) cells were usually isolated as the major population among survivors. Some defective variants of Hfr cells with an intermediate fertility between standard Hfr and F(+) cells had lost sensitivity toward the male-specific ribonucleic acid phage M12. Other defective Hfr variants with as much or less fertility than standard F(+) cells had also all lost sensitivity to phage M12. On single-colony isolation, they segregated nonfertile female H cells which, when infected with F, could restore high fertility with oriented transfer of the chromosome the same as that of the original Hfr H. Also, sensitivity to phage M12 was regained. Female H cells were characterized as those lacking fertility but still retaining a small segment of F or sfa lo...Continue Reading

References

Apr 7, 1967·Biochemical and Biophysical Research Communications·N S Willetts
Mar 1, 1960·Journal of Bacteriology·E A ADELBERG, S N BURNS
Jan 1, 1960·Proceedings of the National Academy of Sciences of the United States of America·Y Hirota
Nov 1, 1952·Genetics·J LederbergE M Lederberg

❮ Previous
Next ❯

Citations

Feb 1, 1976·Japanese Journal of Microbiology·M TomoedaM Hayashi
Jan 1, 1972·Molecular & General Genetics : MGG·S Takahashi, K Matsubara
Jun 11, 1971·Annals of the New York Academy of Sciences·D H Bouanchaud, Y A Chabbert
Jan 1, 1970·Molecular & General Genetics : MGG·J E Dowman, G G Meynell
Jan 1, 1975·Progress in Biophysics and Molecular Biology·M TomoedaT Date
Sep 1, 1975·Antimicrobial Agents and Chemotherapy·H Danbara, M Yoshikawa
Sep 1, 1972·Bacteriological Reviews·R C Clowes
Sep 1, 1991·Journal of Clinical Microbiology·M J AlbertS Tzipori
Aug 1, 1977·Antimicrobial Agents and Chemotherapy·H Danbara, M Yoshikawa
Dec 1, 1974·Journal of Bacteriology·M TomoedaM Konishi
Sep 1, 1982·Journal of Bacteriology·B V Gantotti, S V Beer
Jan 1, 1976·Archives of Biochemistry and Biophysics·M A Lieberman, J S Hong

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.