PMID: 197527Jul 1, 1977Paper

Specific action of T4 endonuclease V on damaged DNA in xeroderma pigmentosum cells in vivo

Proceedings of the National Academy of Sciences of the United States of America
K TanakaY Okada

Abstract

The specific action of T4 endonuclease V on damaged DNA in xeroderma pigmentosum cells was examined using an in vivo assay system with hemagglutinating virus of Japan (Sendai virus) inactivated by UV light. A clear dose response was observed between the level of UV-induce unscheduled DNA synthesis of xeroderma pigmentosum cells and the amount of T4 endonuclease V activity added. The T4 enzyme was unstable in human cells, and its half-life was 3 hr. Fractions derived from an extract of Escherichia coli infected with T4V1, a mutant defective in the endonuclease V gene, showed no ability to restore the UV-induced unscheduled DNA synthesis of xeroderma pigmentosum cells. However, fractions derived from an extract of T4D-infected E. coli with endonuclease V activity were effective. The T4 enzyme was effective in xeroderma pigmentosum cells on DNA damaged by UV light but not in cells damaged by 4-nitroquinoline 1-oxide. The results of these experiments show that the T4 enzyme has a specific action on human cell DNA in vivo. Treatment with the T4 enzyme increased the survival of group A xeroderma pigmentosum cells after UV irradiation.

References

Aug 18, 1976·Biochimica Et Biophysica Acta·S Yasuda, M Sekiguchi
Aug 18, 1976·Biochimica Et Biophysica Acta·Y NishidaM Sekiguchi
Jan 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·B M SutherlandE K Wagner
Jan 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·A R LehmannD Bootsma
Apr 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·U KuhnleinS Linn
Jan 1, 1975·Annual Review of Genetics·J E Cleaver, D Bootsma
Mar 1, 1975·Mutation Research·R S Day
Dec 1, 1975·Mutation Research·K H KraemerD Bootsma
Oct 12, 1973·Biochimica Et Biophysica Acta·H J Edenberg, P C Hanawalt
Sep 23, 1974·Biochimica Et Biophysica Acta·D PapahadjopoulosE Mayhew
Dec 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·S Yasuda, M Sekiguchi

Citations

Mar 1, 1979·Somatic Cell Genetics·T Shiomi, K Sato
Nov 1, 1987·Mutation Research·W K Kaufmann, L P Briley
Jul 1, 1987·Mutation Research·W K Kaufmann, L P Briley
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·C A Smith, P C Hanawalt
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R S Lloyd, P C Hanawalt
Jul 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K ValerieJ K de Riel
May 15, 2008·Expert Opinion on Biological Therapy·Jennifer A Cafardi, Craig A Elmets
Sep 1, 1988·International Journal of Radiation Biology·J S Rubin
Sep 1, 1988·Experimental Cell Research·T Uchida
Jan 1, 1980·Annals of the New York Academy of Sciences·B M Sutherland
Dec 1, 1996·Critical Reviews in Biochemistry and Molecular Biology·A Memisoglu, L Samson
Feb 23, 1978·Nature·R B Setlow
Jan 15, 1981·Nature·P F Lin, F H Ruddle
Jul 31, 1980·Nature·B M Sutherland, S G Hausrath
Dec 1, 1980·European Journal of Pediatrics·C R Bartram

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Glut1 Deficiency

Glut1 deficiency, an autosomal dominant, genetic metabolic disorder associated with a deficiency of GLUT1, the protein that transports glucose across the blood brain barrier, is characterized by mental and motor developmental delays and infantile seizures. Follow the latest research on Glut1 deficiency with this feed.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Nodding Syndrome

Nodding Syndrome is a neurological and epileptiform disorder characterized by psychomotor, mental, and growth retardation. Discover the latest research on Nodding Syndrome here.

LRRK2 & Microtubules

Mutations in the LRRK2 gene are risk-factors for developing Parkinson’s disease (PD). LRRK2 mutations in PD have been shown to enhance its association with microtubules. Here is the latest research.