Specific adhesion to cellulose and hydrolysis of organophosphate nerve agents by a genetically engineered Escherichia coli strain with a surface-expressed cellulose-binding domain and organophosphorus hydrolase.

Applied and Environmental Microbiology
Aijun A WangWilfred Chen

Abstract

A genetically engineered Escherichia coli cell expressing both organophosphorus hydrolase (OPH) and a cellulose-binding domain (CBD) on the cell surface was constructed, enabling the simultaneous hydrolysis of organophosphate nerve agents and immobilization via specific adsorption to cellulose. OPH was displayed on the cell surface by use of the truncated ice nucleation protein (INPNC) fusion system, while the CBD was surface anchored by the Lpp-OmpA fusion system. Production of both INPNC-OPH and Lpp-OmpA-CBD fusion proteins was verified by immunoblotting, and the surface localization of OPH and the CBD was confirmed by immunofluorescence microscopy. Whole-cell immobilization with the surface-anchored CBD was very specific, forming essentially a monolayer of cells on different supports, as shown by electron micrographs. Optimal levels of OPH activity and binding affinity to cellulose supports were achieved by investigating expression under different induction levels. Immobilized cells degraded paraoxon rapidly at an initial rate of 0.65 mM/min/g of cells (dry weight) and retained almost 100% efficiency over a period of 45 days. Owing to its superior degradation capacity and affinity to cellulose, this immobilized-cell system s...Continue Reading

References

Jun 11, 1992·Protein Engineering·J M GreenwoodD G Kilburn
Sep 1, 1993·Protein Engineering·Z AssoulineR A Warren
Jan 1, 1996·Applied Biochemistry and Biotechnology·S C Hung, J C Liao
Apr 1, 1999·Biotechnology and Bioengineering·M LinderT T Teeri
Aug 10, 1999·Biotechnology and Bioengineering·E ShpigelO Shoseyov
Aug 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·G CarrardP Béguin
Feb 15, 2001·Biotechnology Progress·M ShimazuW Chen
Aug 20, 1996·Biotechnology and Bioengineering·K E Lejeune, A J Russell

❮ Previous
Next ❯

Citations

Sep 5, 2009·Applied Microbiology and Biotechnology·Bin CaoKai-Chee Loh
Oct 5, 2010·Applied Microbiology and Biotechnology·Casey M Theriot, Amy M Grunden
Nov 18, 2005·Journal of Industrial Microbiology & Biotechnology·Ayman H ManseeAshok Mulchandani
Jun 7, 2012·The Journal of Physical Chemistry. a·William R CreasyDavid J McGarvey
Aug 6, 2004·Applied and Environmental Microbiology·Brajesh K SinghDenis J Wright
Jan 5, 2006·Applied and Environmental Microbiology·Jen-You Wang, Yun-Peng Chao
Jun 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Oded ShoseyovIlan Levy
Oct 15, 2007·Pakistan Journal of Biological Sciences : PJBS·Afsin SahinFahrettin Arslan
Oct 15, 2007·Pakistan Journal of Biological Sciences : PJBS·Sakda YainoyVirapong Prachayasittikul
Aug 12, 2014·Applied Microbiology and Biotechnology·Jan SchüürmannJoachim Jose
Dec 11, 2007·Biotechnology Advances·M Saleem Zia-ul-Hassan
Apr 6, 2006·FEMS Microbiology Reviews·Brajesh K Singh, Allan Walker
Apr 22, 2003·Annual Review of Biomedical Engineering·Alan J RussellRichard R Koepsel
Nov 24, 2017·Applied Biochemistry and Biotechnology·Lei HanBo Liang
Aug 23, 2006·Critical Reviews in Biotechnology·Meltem Urgun-DemirtasKrishna Pagilla
Apr 19, 2017·Journal of Molecular Recognition : JMR·A AlmeidaD M F Prazeres
Dec 9, 2020·Toxicology Mechanisms and Methods·Jalal HassanA Wallace Hayes

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.