Specific and stable gene transfer to human embryonic stem cells using pseudotyped lentiviral vectors

Stem Cells and Development
Jung-Eun JangDonald B Kohn

Abstract

Genetic modification of human embryonic stem cells (hESCs) is an important tool for understanding and influencing their biologic properties. At the present time, lentiviral vectors pseudotyped with the vesicular stomatitis virus G protein (VSV-G) have been most effective for stable gene transfer to hESCs. However, they also efficiently transduce murine embryonic fibroblasts (MEF), used to support the undifferentiated state of many commonly used hESC lines. Transduction of both the MEF as well as hESCs complicates analyses of gene transfer and expression. We made lentiviral vectors pseudotyped with envelope glycoproteins from retroviruses that have been shown to have more restricted transduction ranges and evaluated their specificity. Lentiviral vectors pseudotyped by the envelopes from either the gibbon ape leukemia virus (GALV) or the RD114 feline endogenous virus (RD114) specifically transduced hESCs to similar extents as VSV-G pseudotyped vectors, but did not transduce MEF. In addition, gene modfication by these pseudotyped lentiviral vectors was stably maintained throughout differentiation of hESCs in vitro. These pseudotyped lentiviral vectors may be valuable tools for efficient, specific and stable gene modification of hE...Continue Reading

References

Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·M P KavanaughA D Miller
Nov 5, 1997·Nature Biotechnology·R ZuffereyD Trono
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·J E RaskoA D Miller
Feb 25, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Michal GroppBenjamin E Reubinoff

❮ Previous
Next ❯

Citations

Mar 16, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Yael StruloviciRonald G Crystal
Sep 11, 2009·Stem Cells and Development·You-Tzung ChenRichard R Behringer
Oct 3, 2009·Tissue Engineering. Part a·Sujoy K DharaSteven L Stice
Sep 26, 2006·Nucleic Acids Research·Philipp KochOliver Brüstle
Aug 7, 2008·Biological Chemistry·Kai MelchiorMarkus Müschen
Feb 4, 2014·Human Gene Therapy Methods·Kristin SchwankeRobert Zweigerdt
Mar 30, 2011·Journal of Cellular Biochemistry·Mirella DottoriStephanie M Hughes
Oct 4, 2006·Gene Therapy·F Yates, G Q Daley
Sep 6, 2017·Human Gene Therapy Methods·Alok V Joglekar, Salemiz Sandoval

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.