PMID: 9524138May 2, 1998Paper

Specific antibodies to the external vestibule of voltage-gated potassium channels block current

The Journal of General Physiology
B Y ZhouX Y Huang

Abstract

Using delayed-rectifier potassium channels as examples, we have designed two specific blockers by generating specific antipeptide antibodies to epitopes in the external vestibules of two channel proteins, Kv1.2 and Kv3.1. These antibodies reduced whole-cell Kv1.2 or Kv3.1 currents in transfected cells and the effect was blocked by the corresponding peptide antigen, but not by control peptides. A control antibody had little effect on Kv1.2 currents and the Kv1.2 blocker antibody had limited effect on other related potassium currents. Furthermore, the Kv1.2 blocking antibody inhibited dendrotoxin binding to Kv1.2 channel proteins in transfected cells. Moreover, using the Kv1.2 blocker antibody, we determined the presence and relative contribution of endogenous Kv1.2 to the overall endogenous K+ currents in NG108 neuronal cells. This guided design of specific channel blockers will facilitate future physiological studies on ion channel functions.

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Citations

Aug 9, 2001·The Journal of Comparative Neurology·W LiT M Perney
Nov 6, 2012·Journal of Medicinal Chemistry·Sharan K BagalNigel A Swain
Feb 6, 2013·Acta Pharmacologica Sinica·Han Sun, Min Li
Oct 8, 2005·Nature Biotechnology·Christopher D Benham
Sep 20, 2005·Nature Biotechnology·Shang-Zhong XuDavid J Beech
Dec 2, 2009·Nature Reviews. Drug Discovery·Heike WulffLuis A Pardo
Aug 14, 2013·The Journal of General Physiology·Jon T SackJames S Trimmer
Oct 17, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Mary K McGahonTim M Curtis
Oct 11, 2007·BMC Biology·Christopher L DouglasChiara Cirelli
Mar 31, 2012·Future Medicinal Chemistry·A WickendenG Erdemli
Nov 19, 2004·Proceedings of the National Academy of Sciences of the United States of America·Haiyan SunMin Li
Dec 17, 2009·Expert Opinion on Investigational Drugs·Jeffrey J Clare
Jul 8, 2008·British Journal of Pharmacology·J NaylorD J Beech
Jul 2, 2008·Annals of the New York Academy of Sciences·Chaira Cirelli, Daniel Bushey
Aug 23, 2005·Journal of Neuroscience Methods·Athina-Myrto ChioniMustafa B A Djamgoz
Jul 28, 2005·Journal of Neuroscience Methods·Mark DallasJim Deuchars
Sep 1, 2018·Current Protocols in Pharmacology·Caroline S ColleyTrevor C I Wilkinson
Apr 21, 2006·American Journal of Physiology. Cell Physiology·Seema Tiwari-WoodruffJeff Bronstein
Aug 22, 2006·American Journal of Physiology. Heart and Circulatory Physiology·William F Jackson
Mar 28, 2008·Expert Review of Molecular Diagnostics·Chuan-Chu ChouNicholas J Murgolo
May 1, 2010·Expert Review of Clinical Pharmacology·Mark L DallasJim Deuchars
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Jan 29, 2003·The Journal of Biological Chemistry·Piotr Koprowski, Andrzej Kubalski
Sep 1, 2019·Bioelectricity·Ileana Hernandez-ResendizLuis A Pardo

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