Specific antibody recognition of rat pituitary adenylate cyclase activating polypeptide receptors

Endocrine
M LiA Arimura

Abstract

Pituitary adenylate cyclase activating polypeptide (PACAP) is a new member of the secretin/VIP family of peptides. The specific receptor for PACAP has been cloned in rat, human, and bovine tissues. The distribution of the transcripts of PACAP receptor genes has been studied in various tissues using in situ hybridization. However, the unavailability of a specific antibody against the PACAP receptor has hampered further study of the expression of receptor proteins. In the present study, rabbit antisera were generated against a synthetic 25-residue peptide corresponding to the C-terminal intracellular domain of the rat PACAP receptor. To validate the specificity of the antisera, CHO cells and cells stably transfected with rat PACAP receptor cDNA were prepared. Using one of these antisera, the membrane and soluble fractions of the transformants were examined by Western blot analysis. Three bands were observed in subcellular fractions from the transfected CHO cells, but no bands were found in similar preparations from the nontransfected cells. A distinct 57-kDa band, which corresponds to the size of cloned rat PACAP receptor, was detected. In addition, a less intense band, larger than 57 kDa, and a very weakly stained band, smaller ...Continue Reading

References

Feb 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P E GottschallA Arimura
Sep 14, 1990·Biochemical and Biophysical Research Communications·T OhtakiM Fujino
Oct 16, 1989·Biochemical and Biophysical Research Communications·A MiyataD H Coy
Apr 1, 1983·Journal of Clinical Pathology·A C Nierwenhuijzen Kruseman
Oct 31, 1994·Annals of the New York Academy of Sciences·A ArimuraD E Brenneman
Dec 30, 1994·Biochemical and Biophysical Research Communications·M SvobodaP Robberecht
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J R Pisegna, S A Wank
Sep 15, 1993·Biochemical and Biophysical Research Communications·M SvobodaJ Christophe
Jun 11, 1996·Biochimica Et Biophysica Acta·H HashimotoA Baba

❮ Previous
Next ❯

Citations

Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·D VaudryH Vaudry
Feb 3, 1999·Annals of the New York Academy of Sciences·M MuroiA Arimura
Feb 24, 2001·Annals of the New York Academy of Sciences·C J ZhouS Shioda

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.