Specific arrangement of alpha-helical coiled coils in the core domain of the bacterial flagellar hook for the universal joint function

Structure
Takashi FujiiKeiichi Namba

Abstract

The bacterial flagellar hook is a short, highly curved tubular structure connecting the rotary motor to the filament acting as a helical propeller. The bending flexibility of the hook allows it to work as a universal joint. A partial atomic model of the hook revealed a sliding intersubunit domain interaction along the protofilament to produce bending flexibility. However, it remained unclear how the tightly packed inner core domains can still permit axial extension and compression. We report advances in cryoEM image analysis for high-resolution, high-throughput structural analysis and a density map of the hook that reveals most of the secondary structures, including the terminal alpha helices forming a coiled coil. The orientations and axial packing interactions of these two alpha helices are distinctly different from those of the filament, allowing them to have a room for axial compression and extension for bending flexibility without impairing the mechanical stability of the hook.

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Citations

Jun 26, 2012·Journal of Bacteriology·Colleen R CourtneyDaniel B Kearns
Jan 18, 2012·Journal of Structural Biology·Elmar BehrmannPawel A Penczek
Nov 26, 2013·Journal of Structural Biology·Ambroise DesfossesCarsten Sachse
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Oct 21, 2016·Scientific Reports·Young-Ho YoonFadel A Samatey
Apr 22, 2017·Scientific Reports·Koichi D HiraokaKeiichi Namba
Jun 7, 2017·Current Biology : CB·Kelly T Hughes
Jan 26, 2017·Nature Communications·Takashi FujiiKeiichi Namba
Nov 29, 2017·Biophysics Reviews·Akio Kitao, Hiroaki Hata
Dec 14, 2017·Biophysics Reviews·Katsumi Imada
Nov 29, 2017·Scientific Reports·Ismaël DuchesneSimon Rainville
Nov 18, 2017·Scientific Reports·Paula V BulierisFadel A Samatey
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May 24, 2017·Journal of Bacteriology·Elías J MongiardiniAníbal R Lodeiro
Apr 24, 2012·Journal of Bacteriology·Mostyn T BrownRichard M Berry
Nov 5, 2016·Nature Communications·Hideyuki MatsunamiFadel A Samatey
Nov 24, 2019·Nature Communications·Takayuki KatoKeiichi Namba
Jul 25, 2019·Biomolecules·Shuichi Nakamura, Tohru Minamino
Oct 2, 2019·Nature Structural & Molecular Biology·Satoshi ShibataMatthias Wolf
Jul 10, 2019·Biomolecules·Yumiko Saijo-HamanoKatsumi Imada
Aug 14, 2019·Nature Chemical Biology·Michael J LynchBrian R Crane
Apr 3, 2018·Biophysics and Physicobiology·Takashi FujiiKeiichi Namba
Jul 18, 2020·F1000Research·Michael C ThompsonJose A Rodriguez
Mar 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Takashi FujiiKeiichi Namba
May 21, 2020·Biomolecules·Laura Camarena, Georges Dreyfus
Nov 4, 2020·Biomolecules·Brittany L Carroll, Jun Liu

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