PMID: 42907Nov 1, 1979

Specific association of neurotransmitter with somatic lysosomes in an identified serotonergic neuron of Aplysia californica

Proceedings of the National Academy of Sciences of the United States of America
J H SchwartzD J Goldberg

Abstract

Injection of [3H]serotonin directly into the cell body of the giant metacerebral neuron (GCN), an identified serotonergic cell in the Aplysia cerebral ganglion, revealed a striking association of the labeled transmitter with large lysosomes. Most of the [3H]serotonin in the neuron is sequestered by lysosomes, which may function as storage depots for the transmitter. This localization is specific: [3H]serotonin was not found in similar lysosomes in the perikaryon of R2, an identified Aplysia cholinergic neuron. [3H]Dopamine, [3H]histamine, and [3H]-N-acetylgalactosamine injected into the serotonergic neuron were not localized in lysosomes. Because of this specificity, it may be possible to use lysosomal binding of labeled transmitter as a radioautographic method for identifying the transmitter type of a nerve cell body. These organelles are lysosomal in origin because they contain acid phosphatase activity. Moreover, examination of isolated GCN cell bodies by fluorescence microscopy revealed the presence of abundant autofluorescent yellow pigment characteristic of lipofuscin. We suggest that [3H]serotonin labels organelles containing membranes that once were integral components of the serotonergic vesicle. Lysosomes might accumu...Continue Reading

References

Nov 1, 1976·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J P Tranzer, J G Richards
Jan 1, 1979·Annual Review of Neuroscience·J H Schwartz
Nov 4, 1977·Brain Research·J E Goldman, J H Schwartz
Mar 1, 1978·Journal of Neurochemistry·K C DasR Katzman
Aug 1, 1976·The Journal of Cell Biology·J E GoldmanJ H Schwartz
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·M EisenstadtJ H Schwartz
Jun 1, 1974·The Journal of Cell Biology·R T AmbronJ H Schwartz
Apr 1, 1969·The Journal of Cell Biology·M M SalpeterE E Salpeter
Oct 1, 1963·The Journal of Comparative Neurology·L SIMPSONR S NISHIOKA

Related Concepts

Acid Phosphatase
Aplysia
Lysosomes
Neurons
Hydroxytryptamine
Neurohormones

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