Specific association of photosystem II and light-harvesting complex II in partially solubilized photosystem II membranes

FEBS Letters
E J BoekemaJ P Dekker

Abstract

In this study, we report the structural characterization of photosystem II complexes obtained from partially solubilized photosystem II membranes. Direct observation by electron microscopy, within a few minutes after a mild disruption of the membranes with the detergent n-dodecyl-alpha,D-maltoside, revealed the presence of several large supramolecular complexes. Images of these complexes were subjected to multivariate statistical analysis and classification procedures, resolving a new complex consisting of the previously characterized dimeric supercomplex of photosystem II and light-harvesting complex II [Boekema et al., Proc. Natl. Acad. Sci. USA 92 (1995) 175-179] and two additional, symmetrically organized protein masses each containing a second type of trimeric light-harvesting II complex. We conclude that large and labile integral membrane proteins, such as photosystem II, can be quickly structurally characterized without extensive purification.

References

Feb 15, 1992·European Journal of Biochemistry·R Bassi, P Dainese
Jan 1, 1988·Methods in Enzymology·G HarauzM van Heel
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·E J BoekemaM Rögner
Feb 8, 1994·Biochimica Et Biophysica Acta·S Jansson
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Ben HankamerEgbert J. Boekema
Jun 1, 1996·Annual Review of Plant Physiology and Plant Molecular Biology·P. HortonR. G. Walters
Jun 1, 1991·Photosynthesis Research·P J van LeeuwenH J van Gorkom

❮ Previous
Next ❯

Citations

Mar 26, 2013·Plant Molecular Biology·Maïna FlorisElodie Lanet
Aug 29, 2009·Photosynthesis Research·Robin Purchase, Silvia Völker
Jul 3, 2010·Photosynthesis Research·Jonathan A D Neilson, Dion G Durnford
Jun 14, 2005·Micron : the International Research and Review Journal for Microscopy·Frantisek VachaMartin Vacha
Aug 17, 1999·Current Opinion in Structural Biology·J Barber, W Kühlbrandt
Dec 29, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J NieldJ Barber
Dec 29, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·P HortonM Wentworth
Nov 14, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Simone BareraJames Barber
Jul 16, 2014·Biochimica Et Biophysica Acta·Sandrine E D'HaeneRaoul N Frese
Mar 28, 2012·The Plant Journal : for Cell and Molecular Biology·Reinat NevoZiv Reich
Apr 28, 2001·Biophysical Journal·V BarzdaL Valkunas
Apr 5, 2015·Micron : the International Research and Review Journal for Microscopy·Natalya V DudkinaEgbert J Boekema
Dec 10, 1999·Biophysical Journal·M L GrootJ P Dekker
Dec 29, 2004·Biochimica Et Biophysica Acta·Jan P Dekker, Egbert J Boekema
Sep 12, 1998·Biochimica Et Biophysica Acta·P KernenZ Krupa
Dec 7, 2017·Plant, Cell & Environment·Alexandra J TownsendAlexander V Ruban
Sep 19, 2002·The Journal of Biological Chemistry·Alexander V RubanPeter Horton
Oct 13, 2018·Biophysical Reports·Xin ShengZhenfeng Liu
Sep 14, 2019·Biochimica Et Biophysica Acta. Bioenergetics·Haijun Liu, Robert E Blankenship
Apr 27, 1999·Biochimica Et Biophysica Acta·F A WollmanR Nechushtai

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.