PMID: 6028046Aug 11, 1967Paper

Specific binding activity of isolated light chains of antibodies

Science
T J YooD Pressman

Abstract

Free light chains isolated from specifically purified antibody have been shown to bind specific hapten. This proves that part of the binding site does exist on the light chain. The light chains were obtained from antibody directed against the 4-azonaphthalene-1-sulfonate group, and the binding of the simple hapten 4-anilinonaphthalene-1-sulfonate was determined by the fluorescence-enhancement technique. Since this hapten undergoes a striking increase in fluorescence on binding to light chains (and also on binding to specific antibody), the presence of small amounts of bound hapten could be determined, even in the presence of the high concentrations of unbound hapten required because of the low binding constant.

References

Oct 1, 1965·The Journal of Experimental Medicine·O A RoholtD Pressman
Dec 1, 1965·Immunochemistry·J W Goodman, J J Donch
Jun 1, 1965·Immunochemistry·K OnoueD Pressman
Jan 1, 1953·Advances in Protein Chemistry·G WEBER
Aug 23, 1963·Science·O A ROHOLTD PRESSMAN
Feb 1, 1962·Journal of Molecular Biology·M WINKLER
Aug 1, 1963·The Biochemical Journal·J B FLEISCHMANE M PRESS
Apr 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·H METZGERS J SINGER
Sep 1, 1964·Biochemistry·S UTSUMI, F KARUSH
Sep 1, 1940·The Biochemical Journal·R A Kekwick

❮ Previous
Next ❯

Citations

Dec 1, 1987·Molecular Immunology·J A LedbetterT T Tsu
Nov 18, 1969·Biochimica Et Biophysica Acta·B R SmithD S Munro
Apr 17, 2003·Trends in Immunology·Frank A Redegeld, Frans P Nijkamp
Jan 8, 1976·The New England Journal of Medicine·A Solomon
Apr 30, 1981·The New England Journal of Medicine·E F Osserman
Dec 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·P VassalliB Benacerraf
May 1, 1973·British Journal of Haematology·G G Glenner
Aug 21, 2007·Applied Microbiology and Biotechnology·M M Harmsen, H J De Haard
Feb 24, 2009·Journal of Neuroimmunology·Anneke RijnierseAletta D Kraneveld
Jun 18, 2002·Nature Medicine·Frank A RedegeldFrans P Nijkamp
Jan 30, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anat BurkovitzYanay Ofran
Jul 20, 2019·Human Antibodies·Manzoor A MirSyed Suhail Hamdani
Sep 1, 1970·Immunochemistry·W B Dandliker, V A De Saussure

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.