Specific binding of dehydroepiandrosterone to the N terminus of the microtubule-associated protein MAP2.

The Journal of Biological Chemistry
Emmanuelle LaurineJean-Michel Verdier

Abstract

The effect of neurosteroids is mediated through their membrane or nuclear receptors. However, no dehydroepiandrosterone (DHEA)-specific receptors have been evidenced so far in the brain. In this paper, we showed by isothermal titration calorimetry that the DHEA specifically binds to the dendritic brain microtubule-associated protein MAP2C with an association constant of 2.7 x 10(7) m-1 and at a molar ratio of 1:1. By partial tryptic digestions and mass spectrometry analysis, we found that the binding involved the N-terminal region of MAP2C. Interestingly, MAP2C displays homologies with 17 beta-hydroxysteroid dehydrogenase 1, an enzyme required for estrogen synthesis. Based on these sequence homologies and on the x-ray structure of the DHEA-binding pocket of 17 beta-hydroxysteroid dehydrogenase 1, we modeled the complex of DHEA with MAP2C. The binding of DHEA to MAP2C involved specific hydrogen bonds that orient the steroid into the pocket. This work suggests that DHEA can directly influence brain plasticity via MAP2C binding. It opens interesting ways for understanding the role of DHEA in the brain.

References

Jan 1, 1991·Methods in Enzymology·J Greer
Jan 1, 1985·Methods of Biochemical Analysis·W C Johnson
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·B Riederer, A Matus
Jan 2, 1986·European Journal of Biochemistry·M A HernándezJ M Andreu
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·C CorpéchotE E Baulieu
Sep 1, 1982·Journal of Ultrastructure Research·W A Voter, H P Erickson
Nov 21, 1995·Proceedings of the National Academy of Sciences of the United States of America·N KalchevaB Shafit-Zagardo
Jan 1, 1994·Trends in Neurosciences·A Matus
Mar 1, 1994·Protein Science : a Publication of the Protein Society·U Hobohm, C Sander
Jul 20, 1993·Journal of Molecular Biology·B Rost, C Sander
Jan 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D Shortle
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·V G KimonidesJ Herbert
Nov 17, 1999·Proceedings of the Society for Experimental Biology and Medicine·A CardounelM Kalimi
Dec 22, 1999·Journal of Molecular Biology·N BlomS Brunak
Mar 29, 2000·Proceedings of the National Academy of Sciences of the United States of America·K MurakamiP Robel
Apr 20, 2000·Neuroscience Research·K TsutsuiM Takase
Dec 18, 2001·Brain Research. Brain Research Reviews·E Plassart-Schiess, E E Baulieu
Mar 14, 2002·Journal of Neuroscience Research·C González-BillaultJ Avila
Aug 7, 2002·The Journal of Cell Biology·Akihiro HaradaNobutaka Hirokawa
Oct 10, 2002·Brain Research Bulletin·Andrea Reyna-NeyraClorinda Arias
Jan 22, 2003·Biochemical and Biophysical Research Communications·Anders MalmendalWalter J Chazin
Feb 20, 2003·Biochemistry·T Chris GamblinLester I Binder

❮ Previous
Next ❯

Citations

Dec 4, 2003·Current Opinion in Pharmacology·Emanuela Binello, Catherine M Gordon
May 8, 2004·Molecular and Cellular Endocrinology·R MindnichJ Adamski
Jun 4, 2011·Biochemistry·C S SzaszP Tompa
May 19, 2004·Proceedings of the National Academy of Sciences of the United States of America·Ioannis CharalampopoulosAchille Gravanis
Sep 18, 2008·Neuroreport·Kaori Mizota, Hiroshi Ueda
Jun 28, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Tuong-Vi NguyenSherif Karama
Jun 4, 2014·The Journal of Steroid Biochemistry and Molecular Biology·N PluchinoA R Genazzani
Jul 22, 2009·Journal of Endocrinological Investigation·G ValentiR S Schwartz
Mar 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Virginie Fontaine-LenoirPaul Robel
Jan 13, 2012·The Journal of Nutrition, Health & Aging·M MaggioG P Ceda
Jul 31, 2010·Molecular and Cellular Endocrinology·Dongmin LiuJoseph S Dillon
Mar 17, 2010·The Journal of Steroid Biochemistry and Molecular Biology·Matheus Parmegiani JahnLuiz Carlos Kucharski
Jun 10, 2009·Frontiers in Neuroendocrinology·Jean Luc Do RegoHubert Vaudry
Dec 10, 2008·Frontiers in Neuroendocrinology·Nicole ManingerSynthia H Mellon
May 14, 2008·Cell Motility and the Cytoskeleton·Carole Abi Farah, Nicole Leclerc
Feb 26, 2016·Journal of Molecular Endocrinology·Russell A ProughCarolyn M Klinge
Sep 24, 2004·Journal of Molecular Recognition : JMR·Matthew J CliffJohn E Ladbury
Jun 22, 2018·The Journal of Biological Chemistry·Kateřina MelkováLukáš Žídek
Nov 20, 2009·Cell Biochemistry and Function·Maria Helena Vianna Metello JacobMaria Flavia Marques Ribeiro
Dec 13, 2019·Biologie aujourd'hui·Nicolas Froger
Mar 19, 2020·Current Alzheimer Research·Dubravka S StracNela Pivac
May 11, 2017·Journal of Neuroendocrinology·T-V Nguyen
Jun 29, 2018·Pharmacology & Therapeutics·Marina SchvererIsabelle Villey
Mar 3, 2005·Neuroscience Letters·Rachel MaayanAbraham Weizman
Aug 10, 2010·The Journal of Steroid Biochemistry and Molecular Biology·Daiane da Rocha JannerMaria Flávia Marques Ribeiro
Aug 13, 2011·The Journal of Steroid Biochemistry and Molecular Biology·Maria Helena Vianna Metello JacobAdriane Belló-Klein
Sep 14, 2010·Experimental Gerontology·Maria Helena Vianna Metello JacobAdriane Belló-Klein

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.