Specific cardiolipin binding interferes with labeling of sulfhydryl residues in the adenosine diphosphate/adenosine triphosphate carrier protein from beef heart mitochondria

Biochemistry
K Beyer, B Nuscher

Abstract

The interaction of cardiolipin with the isolated ADP/ATP carrier protein from beef heart mitochondria has been studied by means of the unmasking of a single cysteinyl residue, Cys56, which accompanies the conformational transition of the protein [Leblanc, P., & Clauser, H, (1972) FEBS Lett. 23, 107-113]. The unmasking was monitored by using the static fluorescence of the sulfhydryl reagent N-(1-pyrenyl)maleimide (PYM). The rate of PYM binding that was observed after initiation of the conformational transition by ADP was drastically reduced in the presence of cardiolipin (CL). Phospholipids other than CL were much less effective. It can be shown that the conformational transition and the binding reaction are both affected by CL, although to varying extents. An enhancement of the rate of the ADP-dependent PYM binding was observed upon digestion of the protein bound phospholipid by phospholipase A2. The phospholipase treatment also led to an increased ADP-independent PYM binding, thus indicating that the ADP control of the carrier transition was gradually lost. The ADP control could be fully restored through the addition of CL, provided that the phospholipase incubation had been terminated after approximately 1 h. These results wi...Continue Reading

References

Apr 1, 1989·Archives of Biochemistry and Biophysics·M Klingenberg
Apr 5, 1983·European Journal of Biochemistry·M KlingenbergH Aquila
Apr 1, 1965·Journal of Biochemistry·H OKUYAMA, S NOJIMA

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Citations

Apr 25, 2007·Apoptosis : an International Journal on Programmed Cell Death·Michal SchwarzAtan Gross
May 9, 2000·Progress in Lipid Research·M SchlameM L Greenberg
Jan 11, 2003·Free Radical Biology & Medicine·Hirotaka Imai, Yasuhito Nakagawa
Sep 19, 2008·Molecular Biology of the Cell·Shuliang ChenMiriam L Greenberg
Oct 21, 2010·Journal of Lipid Research·Junhwan Kim, Charles L Hoppel
May 22, 2013·Journal of Lipid Research·Irina G StavrovskayaBruce S Kristal
Dec 14, 2007·Journal of Lipid Research·Michael Schlame
Jan 2, 2013·Frontiers in Bioscience (Landmark Edition)·Matthew G Baile, Steven M Claypool
Nov 6, 2013·Chemistry and Physics of Lipids·Quan He, Xianlin Han
Jan 22, 2014·Chemistry and Physics of Lipids·Vaishnavi Raja, Miriam L Greenberg
Feb 1, 2011·Journal of Bioenergetics and Biomembranes·Iseli L NantesCarolina G dos Santos
Sep 6, 2012·Heart Failure Reviews·Mariana G Rosca, Charles L Hoppel
Sep 10, 2008·The Journal of Cell Biology·Steven M ClaypoolCarla M Koehler
Dec 26, 2015·Progress in Lipid Research·Stephanie GhioNeville Vassallo
Sep 30, 2015·Chemistry and Physics of Lipids·Gerard J Gaspard, Christopher R McMaster
May 6, 2004·Annals of the New York Academy of Sciences·Yasuhito Nakagawa
Apr 24, 2008·Molecular Microbiology·Shuliang ChenMiriam L Greenberg
May 9, 2009·Biochimica Et Biophysica Acta·Steven M Claypool
Sep 6, 2011·Biochimica Et Biophysica Acta·Kai Stefan Dimmer, Doron Rapaport
Aug 18, 2004·Current Opinion in Structural Biology·Eva Pebay-Peyroula, Gérard Brandolin
Jun 23, 2009·Biochimica Et Biophysica Acta·Martin Klingenberg
Nov 3, 2004·Biochimica Et Biophysica Acta·Hildur Palsdottir, Carola Hunte
Nov 25, 2014·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Nicolas MartinHelga Guderley
Oct 20, 2006·Biochimica Et Biophysica Acta·Yinxi HuangGenxi Li
Oct 18, 2005·Chemistry and Physics of Lipids·Michael SchlameIvan Haller
May 9, 2012·Biochimica Et Biophysica Acta·Rodrigo Arias-CartinAxel Magalon
Apr 19, 2008·Biochimica Et Biophysica Acta·Simon PopeSimon J R Heales
Aug 30, 2008·Biochimica Et Biophysica Acta·Amit S JoshiMiriam L Greenberg
May 31, 2008·Biochimica Et Biophysica Acta·Martin Klingenberg
May 5, 2011·Biophysical Journal·Devrim AcehanMichael Schlame
Aug 11, 2010·Biochimica Et Biophysica Acta·Natalia GebertDiana Stojanovski
Feb 14, 2012·Biophysical Journal·Viktoria BetaneliPetra Schwille
Feb 15, 2003·Biochimica Et Biophysica Acta·M Opekarová, W Tanner
Mar 12, 2013·Chemistry and Physics of Lipids·Elizabeth J Denning, Oliver Beckstein
Jul 30, 2016·Nature Reviews. Endocrinology·Xianlin Han
Aug 16, 2016·Archives of Pharmacal Research·Joseph A Flores-ToroJae-Sung Kim
Jul 2, 2017·Biochimica Et Biophysica Acta. Bioenergetics·Lamia MlayehFabrice Homblé

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