Specific cardiolipin-SecY interactions are required for proton-motive force stimulation of protein secretion

Proceedings of the National Academy of Sciences of the United States of America
Robin A CoreyIan Collinson

Abstract

The transport of proteins across or into membranes is a vital biological process, achieved in every cell by the conserved Sec machinery. In bacteria, SecYEG combines with the SecA motor protein for secretion of preproteins across the plasma membrane, powered by ATP hydrolysis and the transmembrane proton-motive force (PMF). The activities of SecYEG and SecA are modulated by membrane lipids, particularly cardiolipin (CL), a specialized phospholipid known to associate with a range of energy-transducing machines. Here, we identify two specific CL binding sites on the Thermotoga maritima SecA-SecYEG complex, through application of coarse-grained molecular dynamics simulations. We validate the computational data and demonstrate the conserved nature of the binding sites using in vitro mutagenesis, native mass spectrometry, biochemical analysis, and fluorescence spectroscopy of Escherichia coli SecYEG. The results show that the two sites account for the preponderance of functional CL binding to SecYEG, and mediate its roles in ATPase and protein transport activity. In addition, we demonstrate an important role for CL in the conferral of PMF stimulation of protein transport. The apparent transient nature of the CL interaction might fac...Continue Reading

References

Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·C J McKnightL M Gierasch
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·K E McAuleyM R Jones
Oct 25, 2000·Protein Science : a Publication of the Protein Society·A FiserA Sali
Sep 26, 2002·FEBS Letters·Thomas H Haines, Norbert A Dencher
May 6, 2003·Biochimica Et Biophysica Acta·A G Lee
Dec 9, 2003·Nature·Bert Van den BergTom A Rapoport
Nov 3, 2004·Biochimica Et Biophysica Acta·Hildur Palsdottir, Carola Hunte
Apr 5, 2007·Nature Protocols·Helena Hernández, Carol V Robinson
Apr 10, 2007·The Journal of Biological Chemistry·Vicki A M GoldIan Collinson
Jun 5, 2007·The Journal of Physical Chemistry. B·Martin Dahlberg
Jun 16, 2007·The Journal of Physical Chemistry. B·Siewert J MarrinkAlex H de Vries
Jun 23, 2007·Nucleic Acids Research·Eoin FahyShankar Subramaniam
Jul 8, 2008·Journal of Molecular Biology·Dylan B CooperLinda L Randall
May 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Vicki A M GoldIan Collinson
Nov 9, 2010·The Journal of Biological Chemistry·Karine DevilleIan Collinson
May 12, 2012·Cell Reports·Dilem HizlanIan Collinson
Aug 29, 2012·The Journal of Cell Biology·Eunyong Park, Tom A Rapoport
Sep 19, 2012·Proceedings of the National Academy of Sciences of the United States of America·Brandon K TanZiqiang Guan
Mar 9, 2013·Nature Protocols·Arthur LaganowskyCarol V Robinson
May 28, 2013·Chemical Society Reviews·Siewert J Marrink, D Peter Tieleman
Sep 24, 2013·PloS One·Gerd Olofsson, Emma Sparr
Jan 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jonathan J RuprechtEdmund R S Kunji
Aug 25, 2015·Biophysical Journal·Joan Planas-IglesiasJudith Klein-Seetharaman
Sep 16, 2015·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Ian CollinsonWilliam J Allen
Oct 20, 2015·Biology of Sex Differences·Maryline MoulinPierre Chaminade
Feb 11, 2014·Journal of Chemical Theory and Computation·Tsjerk A WassenaarD Peter Tieleman
Jan 8, 2013·Journal of Chemical Theory and Computation·Djurre H de JongSiewert J Marrink
May 11, 2010·Journal of Chemical Theory and Computation·Martin Dahlberg, Arnold Maliniak
Mar 12, 2016·Biochimica Et Biophysica Acta·Murugappan Sathappa, Nathan N Alder
Mar 15, 2016·Structure·Robin A CoreyIan Collinson
Jul 7, 2016·Proceedings of the National Academy of Sciences of the United States of America·Anna L DuncanJohn E Walker

❮ Previous
Next ❯

Citations

Mar 20, 2019·The Journal of Biological Chemistry·Jacob L W MorganWilliam N Zagotta
May 28, 2019·The Protein Journal·Dries SmetsAnastassios Economou
Dec 25, 2019·Biochemical Society Transactions·Robin A CoreyMark S P Sansom
May 27, 2020·Biochemical Society Transactions·Anna L Duncan
Aug 21, 2019·Journal of Bacteriology·Joshua K ChuTimothy R Hoover
Jul 1, 2020·Biochemical Society Transactions·Andy M LauArgyris Politis
Mar 28, 2020·Proceedings of the National Academy of Sciences of the United States of America·Anna L DuncanMark S P Sansom
Feb 13, 2021·Biomolecules·Manuel Halte, Marc Erhardt
Apr 10, 2021·Proceedings of the National Academy of Sciences of the United States of America·Martin V DouglassM Stephen Trent
Oct 16, 2020·The FEBS Journal·Sabrina KochArnold J M Driessen
May 4, 2021·Frontiers in Molecular Biosciences·Julia OswaldHans-Georg Koch
Aug 3, 2019·Microbiology Spectrum·Amalina Ghaisani Komarudin, Arnold J M Driessen
Apr 12, 2019·Journal of the American Society for Mass Spectrometry·Marius M KostelicMichael T Marty
Jul 28, 2021·The FEBS Journal·Michael KamelAlexej Kedrov
Apr 30, 2020·Journal of the American Chemical Society·Chitrak GuptaAbhishek Singharoy
Oct 30, 2020·Analytical Chemistry·James E KeenerMichael T Marty
Sep 3, 2019·Journal of Chemical Theory and Computation·Robin A CoreyPhillip J Stansfeld
Jan 10, 2019·Chemical Reviews·Siewert J MarrinkMark S P Sansom
Aug 22, 2021·Science Advances·Robin A CoreyPhillip J Stansfeld
Feb 14, 2019·Chemical Reviews·Valentina CorradiD Peter Tieleman

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.