PMID: 16507249Mar 2, 2006Paper

Specific coalescent peptide of CD59 screened by phage library

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Ying Cheng, Mei-Hua Gao

Abstract

To screen and identify the short-peptide which specifically bind to human CD59 so as to design short-peptide clamp with counteracting tumor escape activity. Using CHO cells which high express human CD59 as target cells, the phage 12 peptide library was screened for 5 rounds by competitive binding test. After five rounds of screening and competitive binding test, positive phage clones were screened by ELISA and sequenced. 8 out of 16 phage clones which were chosen randomly were identified to have high binding power to CD59. After sequencing, 3 high homologous amino acids were obtained. DNAstar analysis showed that 3 sequences had some homology with the sequence of human CD2 published by PubMed. The obtained sequence HxAxxxxxxPxx is helpful for design of short-peptide clamp of active sites of CD59 related to tumor escape.

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