PMID: 9177217Jun 10, 1997Paper

Specific complex formation between the type II bare lymphocyte syndrome-associated transactivators CIITA and RFX5

Proceedings of the National Academy of Sciences of the United States of America
T SchollJ L Strominger

Abstract

Two of the genes defective in the five complementation groups identified in the class II-negative bare lymphocyte syndrome or corresponding laboratory mutants have been cloned. One gene encodes a protein, RFX5, that is a member of the RFX family of DNA binding proteins. The other, CIITA, encodes a large protein with a defined acidic transcriptional activation domain; this protein does not interact with DNA. Expression plasmids encoding regions of RFX5 fused to the GAL4 DNA binding domain activated transcription from a reporter construct containing GAL4 sites in a cotransfection assay in the Raji human B cell line. However, these plasmids produced transcriptional activity in HeLa cells only in conjunction with interferon gamma stimulation, a condition in which expression of both CIITA and class II major histocompatibility complex surface proteins are induced. Furthermore, these plasmids were not active in RJ2.2.5, an in vitro mutagenized derivative of Raji in which both copies of CIITA are defective. Transcriptional activation by the RFX5 fusion protein could be restored in RJ2.2.5 by cotransfection with a CIITA expression plasmid. Finally, a direct interaction between RFX5 and CIITA was detected with the yeast two-hybrid and fa...Continue Reading

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Citations

Jan 22, 2002·International Journal of Cancer. Journal International Du Cancer·Nienke van der StoepPeter J van den Elsen
Jul 17, 1998·Immunology Today·P J van den ElsenS J Gobin
Oct 2, 2001·Neurochemistry International·E N BenvenisteG M O'Keefe
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Dec 30, 1999·Microbes and Infection·J D FontesB M Peterlin
Feb 5, 2008·The Journal of Biological Chemistry·Lilien N VoongDrew E Cressman
Nov 13, 2001·Medicine·J VillardW Reith
Jun 14, 2000·Journal of Virology·Y C PengE J Androphy
Aug 11, 2000·Molecular and Cellular Biology·J A Harton, J P Ting
Aug 11, 2000·Molecular and Cellular Biology·A M DeSandroJ M Boss
Jul 5, 2001·Molecular and Cellular Biology·T J SiskC H Chang
Mar 13, 2001·Annual Review of Immunology·W Reith, B Mach
Aug 19, 2007·Biological Chemistry·Srikarthika Jambunathan, Joseph D Fontes
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·K L WrightJ P Ting
Jan 25, 2003·Tissue Antigens·M T CoirasM Sánchez-Pérez
May 12, 2007·Journal of Molecular Biology·Wafa Al-KandariJoseph D Fontes
Apr 8, 2006·Molecular Immunology·Wafa Al-KandariJoseph D Fontes
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Oct 3, 2014·Antioxidants & Redox Signaling·Nathan A BraceyDaniel A Muruve
Apr 19, 2016·BioFactors·Isaac DownsKoichi S Kobayashi

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