PMID: 6402035Jan 4, 1983Paper

Specific detection of N-acetylglucosamine-containing oligosaccharide chains on ovine submaxillary asialomucin

Biochimica Et Biophysica Acta
D H van den EijndenE G Berger

Abstract

Human milk beta-N-acetylglucosaminide beta 1 leads to 4-galactosyltransferase (EC 2.4.1.38) was used to galactosylate ovine submaxillary asialomucin to saturation. The major [14C]galactosylated product chain was obtained as a reduced oligosaccharide by beta-elimination under reducing conditions. Analysis by Bio-Gel filtration and gas-liquid chromatography indicated that this compound was a tetrasaccharide composed of galactose, N-acetylglucosamine and reduced N-acetylgalactosamine in a molar ratio of 2:0.9:0.8. Periodate oxidation studies before and after mild acid hydrolysis in addition to thin-layer chromatography revealed that the most probable structure of the tetrasaccharide is Gal beta 1 leads to 3([14C]Gal beta 1 leads to 4GlcNAc beta 1 leads to 6)GalNAcol. Thus it appears that Gal beta 1 leads to 3(GlcNAc beta 1 leads to 6)GalNAc units occur as minor chains on the asialomucin. The potential interference of these chains in the assay of alpha-N-acetylgalactosaminylprotein beta 1 leads to 3-galactosyltransferase activity using ovine submaxillary asialomucin as an acceptor can be counteracted by the addition of N-acetylglucosamine.

References

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Jul 5, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Xiaohui SunJianmin Cui

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Citations

Jan 1, 1986·CRC Critical Reviews in Biochemistry·G J Strous
Dec 15, 1986·International Journal of Cancer. Journal International Du Cancer·J Van RinsumD H Van den Eijnden
Feb 1, 1984·European Journal of Biochemistry·D StojanovicR C Hughes

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