PMID: 9171406Jun 1, 1997Paper

Specific detection of Salmonella typhimurium proteins synthesized intracellularly

Journal of Bacteriology
L L Burns-KeliherR Curtiss

Abstract

Studies of the proteins Salmonella typhimurium synthesizes under conditions designed to more closely approximate the in vivo environment, i.e., in cell and tissue culture, are not easily interpreted because they have involved chemical inhibition of host cell protein synthesis during infection. The method which we have developed allows specific labeling of bacterial proteins without interfering with host cell metabolic activities by using a labeled lysine precursor which mammalian cells cannot utilize. We have resolved the labeled proteins using two-dimensional electrophoresis and autofluorography. We were able to detect 57 proteins synthesized by S. typhimurium during growth within a human intestinal epithelial cell line. Of the 57 proteins detected, 34 appear to be unique to the intracellular environment, i.e., they are not seen during growth of the bacteria in tissue culture medium alone. Current (and future) efforts are directed at organizing the 34 proteins into known stress response groups, determining the cellular locations of the proteins (outer or inner membrane, etc.), and comparing the pattern of proteins synthesized within an intestinal epithelial cell to the pattern synthesized during growth within other tissues.

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Citations

Apr 29, 2000·Electrophoresis·P Cash
Sep 24, 1999·Electrophoresis·Y A Kwaik, O S Harb
Jun 30, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·I Hautefort, J C Hinton
Nov 10, 2006·Proceedings of the National Academy of Sciences of the United States of America·Andrea J McCoyAnthony T Maurelli
Jun 30, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·H Smith
Feb 7, 1998·Infection and Immunity·L Burns-KeliherR Curtiss
Dec 22, 1999·Microbes and Infection·O S Harb, Y Abu Kwaik
Feb 3, 1998·Trends in Microbiology·D M HeithoffM J Mahan
Apr 7, 2004·Pathologie-biologie·V FoulongneM Ramuz

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