Specific determination of selenoaminoacids in whole milk by 2D size-exclusion-ion-paring reversed phase high-performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP MS)

Analytica Chimica Acta
Katarzyna BierlaRyszard Lobinski

Abstract

A procedure was developed for the quantitative recovery of selenomethionine (SeMet) and selenocysteine (SeCys) from whole milk. It was based on the protein unfolding, carbamidomethylation of the aminoacid residues using iodoacetamide and proteolysis using Protease XIV. The selenoaminoacids were specifically determined by ion-paring reversed phase HPLC-ICP MS after their isolation from the post-reaction mixture by size-exclusion LC. Se(IV) present in the sample was derivatized as well and was determined along with the selenoaminoacids. The origin and identity of species were identified by the co-elution with the Se(IV), isotopically labelled selenomethionine, and with the synthetic standard of carbamidomethylated selenocysteine. The method development for SeCys was assisted by using glutathione peroxidase as the SeCys standard. SeMet, SeCys and Se(IV) were quantified by the method of standard additions. The mass balance provided a measure of the method validation. The method was applied to monitoring selenium speciation during supplementation of cows (dose-effect study) with Se-rich yeast containing feed and during milk processing.

References

Dec 1, 1988·Biological Trace Element Research·I D BhattacharyaJ A Milner
Sep 1, 1987·The Science of the Total Environment·Y Hojo

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Citations

Oct 19, 2010·Analytical and Bioanalytical Chemistry·Bente GammelgaardCharlotte Gabel-Jensen
Jul 6, 2014·Food Chemistry·María PalomoYolanda Madrid
Jan 13, 2012·Journal of Proteomics·Yu-Dong WangYum-shing Wong
Oct 10, 2013·Journal of Separation Science·Cui YangDonghao Li
Feb 15, 2018·International Journal of Molecular Sciences·Katarzyna BierlaJoanna Szpunar

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