PMID: 15240691Jul 9, 2004Paper

Specific engagement of TLR4 or TLR3 does not lead to IFN-beta-mediated innate signal amplification and STAT1 phosphorylation in resident murine alveolar macrophages

The Journal of Immunology : Official Journal of the American Association of Immunologists
Antonello PunturieriJeffrey L Curtis

Abstract

The innate immune response must be mobilized promptly yet judiciously via TLRs to protect the lungs against pathogens. Stimulation of murine peritoneal macrophage (PMphi) TLR4 or TLR3 by pathogen-associated molecular patterns (PAMPs) typically induces type I IFN-beta, leading to autocrine activation of the transcription factor STAT1. Because it is unknown whether STAT1 plays a similar role in the lungs, we studied the response of resident alveolar macrophages (AMphi) or control PMphi from normal C57BL/6 mice to stimulation by PAMPs derived from viruses (polyriboinosinic:polyribocytidylic acid, specific for TLR3) or bacteria (Pam(3)Cys, specific for TLR2, and repurified LPS, specific for TLR4). AMphi did not activate STAT1 by tyrosine phosphorylation on Y701 following stimulation of any of these three TLRs, but readily did so in response to exogenous IFN-beta. This unique AMphi response was not due to altered TLR expression, or defective immediate-early gene response, as measured by expression of TNF-alpha and three beta chemokines. Instead, AMphi differed from PMphi in not producing bioactive IFN-beta, as confirmed by ELISA and by the failure of supernatants from TLR-stimulated AMphi to induce STAT1 phosphorylation in PMphi. Co...Continue Reading

References

Mar 1, 1995·The Journal of Experimental Medicine·Y YaegashiM A Freudenberg
Oct 14, 1998·American Journal of Respiratory and Critical Care Medicine·S GiosuéF Ameglio
Jun 11, 1999·The Journal of Biological Chemistry·M M MonickG W Hunninghake
Jun 29, 1999·Cold Spring Harbor Symposia on Quantitative Biology·T ManiatisM G Wathelet
Mar 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S AkashiK Miyake
Feb 7, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·J E DurbinD E Levy
Jul 6, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M HirschfeldJ J Weis
Aug 5, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·B HuJ L Curtis
Oct 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M M MonickG W Hunninghake
Sep 6, 2001·Respiration Physiology·D A Schwartz
Mar 16, 2002·Nature Immunology·Vladimir ToshchakovStefanie N Vogel
Apr 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·Brigitte G DornerRichard A Kroczek
May 16, 2002·Chest·Teresa D Tetley
Jun 20, 2002·The Journal of Biological Chemistry·Martha M MonickGary W Hunninghake
Aug 9, 2002·The European Respiratory Journal·M M Monick, G W Hunninghake
Sep 5, 2002·Nature Reviews. Molecular Cell Biology·David E Levy, J E Darnell
Oct 2, 2002·Immunity·Sean DoyleGenhong Cheng
Oct 3, 2002·Annual Review of Medicine·Peter J Barnes
Jan 16, 2003·Immunology Letters·Shizuo Akira, Hiroaki Hemmi
Feb 19, 2003·Nature Genetics·Stéphanie DupuisJean-Laurent Casanova
Apr 8, 2003·Nature Immunology·Marina KaraghiosoffMathias Müller
Apr 11, 2003·The Journal of Biological Chemistry·Sang Hoon RheeMatthew J Fenton
Apr 23, 2003·American Journal of Respiratory Cell and Molecular Biology·Jeffrey L Curtis, Antonello Punturieri
May 30, 2003·Biochemical Society Transactions·S AkiraK Takeda
Jun 18, 2003·Trends in Immunology·Luke A J O'NeillAndrew G Bowie
Sep 10, 2003·Journal of Leukocyte Biology·B BeutlerR J Ulevitch
Sep 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·Kenya HondaTadatsugu Taniguchi
Oct 1, 2003·The Journal of Experimental Medicine·Katherine A FitzgeraldDouglas T Golenbock

❮ Previous
Next ❯

Citations

Mar 8, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mark A RivieccioCelia F Brosnan
Jun 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Antonello PunturieriJeffrey L Curtis
Sep 27, 2006·Infection and Immunity·Tina Mahieu, Claude Libert
Jan 5, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Aranya BagchiJudith Hellman
Sep 18, 2007·American Journal of Respiratory Cell and Molecular Biology·Gordon J GaschlerMartin R Stämpfli
Oct 24, 2009·American Journal of Respiratory and Critical Care Medicine·Thomas H SissonRichard H Simon
May 31, 2016·Mediators of Inflammation·C PomaraE Turillazzi
Dec 2, 2006·American Journal of Respiratory and Critical Care Medicine·Ian SabroeMoira K B Whyte
Jul 16, 2009·The Journal of Clinical Investigation·Francis J MartinAlice Prince
Apr 12, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Famke AeffnerIan C Davis
Apr 28, 2005·Laboratory Investigation; a Journal of Technical Methods and Pathology·Yasushi TakiiHiromi Ishibashi
Jun 24, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Uma M NagarajanToni Darville
Jan 23, 2008·Allergology International : Official Journal of the Japanese Society of Allergology·Yasuhiro Gon
Jun 26, 2012·Journal of Leukocyte Biology·Akira AsaiFujio Suzuki
Aug 8, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Carlos E O BaleeiroRobert Paine
Feb 19, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Michael P MendezRobert Paine
Aug 20, 2014·Communicative & Integrative Biology·Vicky Sender, Cordula Stamme
Aug 29, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Shawn J SkerrettAdeline M Hajjar
Sep 9, 2010·Cell and Tissue Research·David SchnebergerBaljit Singh
Aug 8, 2015·Journal of Cellular Physiology·Alessandra MiceraStefano Bonini
Dec 5, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Crystal L LovingRandy E Sacco
Jul 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Martha M MonickGary W Hunninghake

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.