PMID: 38257May 1, 1979

Specific fluorescent labeling of chicken myofibril Z-line proteins catalyzed by guinea pig liver transglutaminase

The Journal of Cell Biology
D L Gard, E Lazarides

Abstract

Guinea pig liver transglutaminase has been found to catalyze the covalent incorporation of dansylcadaverine into chicken skeletal muscle myofibril proteins. Epifluorescence microscopy reveals that the incorporated dansylcadaverine is specifically localized at or near the myofibril Z line. SDS-polyacrylamide gel electrophoresis (SDS-PAGE) indicates that actin constitutes a major fraction of the labeled material; the Z-line proteins alpha-actinin and desmin also show significant labeling, as well as tropomyosin, several additional unidentified proteins, and material with an extremely high molecular weight. The Z-line-specific fluorescence can be removed by brief trypsinization, which releases fluorescent alpha-actinin into the supernate. The majority of the fluorescent protein species are resistant to extraction by either 0.6 M KCl or KI. These results, in conjunction with the microscopic localization, suggest that the dansyl-labeled proteins are constituents of the myofibril Z line. A significant amount of fluorescently labeled transglutaminase is also present in labeled myofibrils, which is resistant to extraction with either 0.6 M KCl or KI. This result indicates a strong, noncovalent interaction between the transglutaminase m...Continue Reading

References

Sep 9, 1977·Biochemical and Biophysical Research Communications·P J BirckbichlerM K Patterson
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R M Evans, L M Fink
Jul 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·A Dutton, S J Singer
Jan 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·B P LaneE Drummond
Jan 1, 1976·The Journal of Cell Biology·J D EtlingerD A Fischman
Apr 1, 1972·The Anatomical Record·D E Kelly, M A Cahill
Jan 2, 1973·Biochimica Et Biophysica Acta·P J BirckbichlerA G Loewy
Feb 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·S I Chung, J E Folk
Dec 8, 1972·Annals of the New York Academy of Sciences·S I Chung
Feb 1, 1973·Analytical Biochemistry·G R Schacterle, R L Pollack
Jan 1, 1969·The Journal of Cell Biology·M H StromerR V Rice
Aug 1, 1957·Biochimica Et Biophysica Acta·N K SARKARH WAELSCH

Citations

Jan 1, 1985·Annals of the New York Academy of Sciences·P Traub
May 1, 1981·The American Journal of Anatomy·B E Batten, E Anderson
Dec 17, 2014·Analytical Chemistry·Thillai V SekarRamasamy Paulmurugan
Aug 1, 1994·The Canadian Journal of Neurological Sciences. Le Journal Canadien Des Sciences Neurologiques·A G LacsonS Kuhn

Related Concepts

BioDex 1
Dansyl Compounds
Gamma-glutamyl transferase
Liver
Muscle Proteins
Muscle
Myofilaments

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