Specific functions of Drosophila amyloid precursor-like protein in the development of nervous system and nonneural tissues

Journal of Neurobiology
Yan LiAike Guo

Abstract

Drosophila amyloid precursor-like protein (APPL) is expressed extensively in the nervous system soon after neuronal differentiation. By utilizing different transgenic flies, we studied the physiological function of two APPL protein forms, membrane-bound form (mAPPL) and secreted form (sAPPL), in neural development. We found that neither deletion nor overexpression of APPL protein altered the gross structure of mushroom bodies in the adult brain. No changes were detected in cell types and their relative ration in embryo-derived cultures from all APPL mutants. However, the neurite length was significantly increased in mutants overexpressing mAPPL. In addition, mutants lacking sAPPL had numerous neurite branches with abnormal lamellate membrane structures (LMSs) and blebs, while no apoptosis was detected in these neurons. The abnormal neurite morphology was most likely due to the disorganization of the cytoskeleton, as shown by double staining of actin filaments and microtubules. Electrophysiologically, A-type K+ current was significantly enhanced, and spontaneous excitatory postsynaptic potentials (sEPSPs) were greatly increased in APPL mutants lacking sAPPL. Moreover, panneural overexpression of different forms of APPL protein g...Continue Reading

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Citations

Sep 14, 2011·Experimental Brain Research·Burkhard PoeckDoris Kretzschmar
Apr 5, 2007·Cell and Tissue Research·Xinping ChenAike Guo
Mar 31, 2010·Proceedings of the National Academy of Sciences of the United States of America·Hsueh-Cheng ChiangYi Zhong
May 10, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Garrett G GrossMing Guo
May 8, 2013·Small GTPases·Natalia MoraFlorenci Serras
Aug 11, 2016·Frontiers in Molecular Neuroscience·Marlène Cassar, Doris Kretzschmar
Apr 1, 2009·Cellular and Molecular Life Sciences : CMLS·Kristin T Jacobsen, Kerstin Iverfeldt
Dec 23, 2016·Frontiers in Molecular Neuroscience·Thomas Preat, Valérie Goguel
Mar 23, 2010·Neurobiology of Disease·Jill Wentzell, Doris Kretzschmar

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