Specific G protein activation and mu-opioid receptor internalization caused by morphine, DAMGO and endomorphin I

European Journal of Pharmacology
N T BurfordW Sadée

Abstract

Previous studies have shown that the agonist [D-Ala2, N-Me-Phe4, Gly-ol5]enkephalin (DAMGO) but not morphine induces mu-opioid receptor internalization [Arden, J.R., Segredo, V., Wang, Z., Lameh, J., Sadee, W., 1995. J. Neurochem. 65, 1636-1645]. In the present study we investigated the relationship between internalization of the mu-opioid receptor and the specific G proteins activated following treatment with morphine, DAMGO and endomorphin I (Tyr-Pro-Trp-Phe-NH2) (a putative endogenous mu-opioid receptor agonist) in human embryonic kidney (HEK) cells. Endomorphin I and DAMGO, but not morphine, caused mu-opioid receptor internalization. Morphine, DAMGO and endomorphin I each activated Gi1 alpha/Gi2 alpha, Go alpha and Gi3 alpha to a similar extent, but not Gq alpha/G11 alpha or Gs alpha in HEK membranes. Therefore, the three ligands tested differed in their ability to internalize mu-opioid receptors even though they were similar in activating individual G proteins.

References

May 7, 1990·Biochimica Et Biophysica Acta·L BirnbaumerA M Brown
Jul 19, 1996·The Journal of Biological Chemistry·L M Tolbert, J Lameh
Aug 9, 1996·The Journal of Biological Chemistry·D E KeithM von Zastrow
Jan 1, 1997·Annual Review of Pharmacology and Toxicology·D E Clapham, E J Neer
Jun 1, 1997·Journal of Neurochemistry·V SegredoW Sadée

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Citations

Jul 7, 2005·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·Mellar P DavisRuth Lagman
Apr 29, 2005·Brain Research. Molecular Brain Research·Sumita ChakrabartiAlan R Gintzler
Nov 8, 2001·European Journal of Pharmacology·R Przewłocki, B Przewłocka
Mar 17, 1999·European Journal of Pharmacology·B PrzewłockaR Przewłocki
Mar 20, 2002·Pharmacology, Biochemistry, and Behavior·Benedict A GomesByron C Yoburn
Aug 26, 2003·Advanced Drug Delivery Reviews·Ronald G Wiley, Douglas A Lappi
Oct 1, 2012·Journal of Developmental Origins of Health and Disease·Z Y OngB S Muhlhausler
Nov 18, 2000·British Journal of Pharmacology·C HarrisonD G Lambert
Jul 16, 1999·Clinical and Experimental Pharmacology & Physiology·M Connor, M D Christie
Aug 10, 1999·Neuroreport·J C MarvizónE A Mayer
Sep 25, 2001·Neuroreport·C Abbadie, G W Pasternak
Feb 17, 2000·Annals of the New York Academy of Sciences·R PrzewłockiG Toth
May 4, 2012·Molecular Pharmacology·Guadalupe RiveroEamonn Kelly
Oct 7, 2004·European Journal of Pharmacology·Ryszard Przewlocki
Apr 4, 2017·ACS Chemical Neuroscience·Michael RemesicYeon Sun Lee

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