PMID: 698819Oct 6, 1978Paper

Specific glycine uptake by identified neurons of Aplysia californica. I. Autoradiography

Brain Research
C H PriceD J McAdoo

Abstract

The identified giant neurons R3-R14 in the Aplysia parietovisceral ganglion (PVG) have a rapid, Na+-dependent and Hg2+-sensitive uptake system for glycine not found in neighboring neurons. In autoradiographs of PVG incubated in [3H]glycine (glutaraldehyde fix), the cytoplasms of R3-R14 have 3--4 times more silver grains (No./100 sq.micrometer) than other neurons. The glycine uptake system in R3-R14 is selective (alanine, serine, leucine, and proline are taken up equally by all neurons) and is unaffected by reserpine and anisomycin. Neurons R3-R14 contain 2 times less label when ganglia are fixed in formaldehyde than when glutaraldehyde is used as a fixative. Because formaldehyde fixes free amino acids poorly, much of the glycine taken up by R3-R14 is, therefore, not incorporated into protein. In autoradiographs of PVG incubated in [3H]glycine, silver grains are distributed randomly throughout the cytoplasm and nucleus of R3-R14; no association of the grains with the dense core granules characteristic of these neurons7 or other cellular components was found. In contrast, grains in the neurosecretory "bag cells" of the PVG were clustered in numerous discrete areas of the cytoplasm (Golgi complex areas) and the nucleus was only sp...Continue Reading

References

Jan 1, 1976·Journal of Neuroscience Research·F A Henn
Apr 1, 1967·The Journal of Cell Biology·T Peters, C A Ashley
Dec 31, 1973·Zeitschrift für Zellforschung und mikroskopische Anatomie·R Anwyl, L H Finlayson
Jun 1, 1969·The Journal of Cell Biology·S RudeL S Van Orden
Aug 1, 1973·Journal of Neurochemistry·J S De Belleroche, H F Bradford
Mar 1, 1972·Journal of Neurochemistry·J S De Belleroche, H F Bradford
Nov 1, 1965·Life Sciences·M H Aprison, R Werman
Oct 1, 1952·The Biochemical Journal·P R LEWIS

Citations

Mar 1, 1984·Cellular and Molecular Neurobiology·M J McCreery, D O Carpenter
Sep 1, 1984·Cellular and Molecular Neurobiology·K E CarlsonE Aizenman
Oct 1, 1981·Journal of Neurocytology·J T HaskinsJ E Blankenship
Jan 1, 1989·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·C Y LinD J McAdoo
Oct 15, 1980·The Journal of Comparative Neurology·Y C KongD M Lam
Nov 1, 1985·The Journal of Comparative Neurology·C Matute, P Streit

Related Concepts

Metazoa
Aplysia
Autoradiography
Cell Nucleus
Protoplasm
Ganglia
Glycine, Monopotasssium Salt
Intracellular Fluid
Nerve Tissue Proteins
Neurons

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